Molecules, Vol. 26, Pages 5815: Effect of Cold Atmospheric Plasma Jet Associated to Polyene Antifungals on Candida albicans Biofilms

Molecules, Vol. 26, Pages 5815: Effect of Cold Atmospheric Plasma Jet Associated to Polyene Antifungals on Candida albicans Biofilms Molecules doi: 10.3390/molecules26195815 Authors: Lady Daiane Pereira Leite Maria Alcionéia Carvalho de Oliveira Mariana Raquel da Cruz Vegian Aline da Graça Sampaio Thalita Mayumi Castaldelli Nishime Konstantin Georgiev Kostov Cristiane Yumi Koga-Ito The increasing incidence of antifungal resistance represents a great challenge in the medical area and, for this reason, new therapeutic alternatives for the treatment of fungal infections are urgently required. Cold atmospheric plasma (CAP) has been proposed as a promising alternative technique for the treatment of superficial candidiasis, with inhibitory effect both in vitro and in vivo. However, little is known on the association of CAP with conventional antifungals. The aim of this study was to evaluate the effects of the association between CAP and conventional polyene antifungals on Candida albicans biofilms. C. albicans SC 5314 and a clinical isolate were used to grow 24 or 48 h biofilms, under standardized conditions. After that, the biofilms were exposed to nystatin, amphotericin B and CAP, separately or in combination. Different concentrations of the antifungals and sequences of treatment were evaluated to establish the most effective protocol. Biofilms viability after the treatments was compared to negative control. Data were compared by One-way ANOVA and post hoc T...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research