Molecules, Vol. 26, Pages 5815: Effect of Cold Atmospheric Plasma Jet Associated to Polyene Antifungals on Candida albicans Biofilms
Molecules, Vol. 26, Pages 5815: Effect of Cold Atmospheric Plasma Jet Associated to Polyene Antifungals on Candida albicans Biofilms
Molecules doi: 10.3390/molecules26195815
Authors:
Lady Daiane Pereira Leite
Maria Alcionéia Carvalho de Oliveira
Mariana Raquel da Cruz Vegian
Aline da Graça Sampaio
Thalita Mayumi Castaldelli Nishime
Konstantin Georgiev Kostov
Cristiane Yumi Koga-Ito
The increasing incidence of antifungal resistance represents a great challenge in the medical area and, for this reason, new therapeutic alternatives for the treatment of fungal infections are urgently required. Cold atmospheric plasma (CAP) has been proposed as a promising alternative technique for the treatment of superficial candidiasis, with inhibitory effect both in vitro and in vivo. However, little is known on the association of CAP with conventional antifungals. The aim of this study was to evaluate the effects of the association between CAP and conventional polyene antifungals on Candida albicans biofilms. C. albicans SC 5314 and a clinical isolate were used to grow 24 or 48 h biofilms, under standardized conditions. After that, the biofilms were exposed to nystatin, amphotericin B and CAP, separately or in combination. Different concentrations of the antifungals and sequences of treatment were evaluated to establish the most effective protocol. Biofilms viability after the treatments was compared to negative control. Data were compared by One-way ANOVA and post hoc T...
Source: Molecules - Category: Chemistry Authors: Lady Daiane Pereira Leite Maria Alcion éia Carvalho de Oliveira Mariana Raquel da Cruz Vegian Aline da Gra ça Sampaio Thalita Mayumi Castaldelli Nishime Konstantin Georgiev Kostov Cristiane Yumi Koga-Ito Tags: Article Source Type: research