Identification of SRSF3 target mRNAs using inducible TRIBE

In this study, we used inducible TRIBE (targets of RNA binding sites by editing) to identify RNA targets and binding motifs of SRSF3 in human embryonic stem cells (hESCs). We identified 3888 confident binding sites of SRSF3, corresponding to 1222 gene targets. Our results showed that nearly half of the binding sites were distributed in exons, reflecting the alternative splicing function of SRSF3. Motif analysis demonstrated that two of the SRSF3 recognition sequences were the same as the motifs identified in mouse embryonic stem cells, suggesting the recognition sequences of SRSF3 may be conserved in mammals. Overall, our analyses revealed the RNA targets of SRSF3 and uncovered its RNA recognition specificity, providing a valuable resource for understanding the function of SRSF3 in human embryonic stem cells.PMID:34534741 | DOI:10.1016/j.bbrc.2021.09.019

Source: Biochemical and Biophysical Research communications - September 17, 2021 Category: Biochemistry Authors: Siqian Jin Ziwei Xue Jinchun Zhang Ziqi Wang Jin Zhang Di Chen Wanlu Liu Junxin Lin Source Type: research