GSE154333 Cell size is a determinant of stem cell potential during aging (HSC size-fractionation RNA-seq dataset)

Contributors : Jette Lengefeld ; Chia-Wei Cheng ; Pema Maretich ; Marguerite Blair ; Hannah Hagen ; Melanie R McReynolds ; Emily Sullivan ; Kyra Majors ; Christina Roberts ; Joon H Kang ; Joachim D Steiner ; Teemu P Miettinen ; Scott R Manalis ; Adam Antebi ; Sean J Morrison ; Jacqueline A Lees ; Laurie A Boyer ; Ömer H Yilmaz ; Angelika AmonSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusStem cells are remarkably small in size. Human hematopoietic stem cells (HSCs) measure a mere 7  μm in diameter. Whether small size is important for stem cell function is unknown. We find that murine HSCs enlarge under conditions known to decrease stem cell function. This decreased fitness of large HSCs is due to reduced proliferative potential. We further show that preventing HSC enlargemen t by inhibiting macromolecule biosynthesis or reducing the size of large HSCs by shortening G1 averts the loss of stem cell potential. Naturally large HSCs also exhibit decreased stem cell potential indicating that large size characterizes exhausted HSCs under physiological conditions. Finally, we s how that our findings are relevant to aging. A fraction of murine and human HSCs enlarge during aging. Preventing this age-dependent enlargement improves HSC function. We conclude that small cell size is important for stem cell function and propose that stem cell enlargement contributes to their fun ctional decline during aging.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research