GSE165794 Time series (T= 0-4 hours) for E. coli K-12 MC4100 after a shift from 23 ˚C to 37˚C (M9 minimal glycerol medium, exponential phase growth)

Contributor : Christine A White-ZieglerSeries Type : Expression profiling by arrayOrganism : Escherichia coliWe investigated the effect of a temperature shift from 23 ˚C to 37˚C, mimicking the temperature transition experienced by a microbe as it enters a human host. This strategy allowed the exploration of the kinetics of gene expression changes in immediate response to a temperature upshift and to characterize the suite of genes required to respond to 37˚C in the first few hours. We demonstrate that a significant number of genes are rapidly altered in expression within minutes to hours after a temperature shift. Some are only induced transiently, while are required for long-term adaptation. Many of these genes are related to metabolism, stress resp onse, and immune evasion suggesting that temperature may serve as a sentinel cue to predict and prepare for niche adaptation in the host.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by array Escherichia coli Source Type: research

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In this study, the model of the haemolymph node was established by transferring the allogeneic renal tissue in the rat. Intrasinusoidal erythrocytes of the node were the component for producing a red macroscopic appearance, while macrophage-erythrocyte-lymphocyte rosettes were the major immunomorphological changes, which reflects the immune activity against the invasion of the allogeneic tissue within the node. Therefore the haemolymph node is an immunomorphological organ.
Source: Cells Tissues Organs - Category: Cytology Source Type: research
This article is protected by copyright. All rights reserved.PMID:34694043 | DOI:10.1002/cbin.11715
Source: Cell Biology International - Category: Cytology Authors: Source Type: research
ConclusionThe recommended IV dose of 10  mg/kg q8h, not exceeding 400 mg q8h, would achieve AUC0-24  h to successfully treat bacteria with MICs  ≤ 0.25 (e.g. Salmonella, Escherichia coli, Proteus, Haemophilus, Enterobacter, and Klebsiella). A dose increase to 600 mg q8h in children >  40 kg and to 15 mg/kg q8h (max 400 mg q8h, max 600 mg q8h if augmented renal clearance, i.e., eGFR >  200 mL/min/1.73 m2) in children  
Source: European Journal of Clinical Pharmacology - Category: Drugs & Pharmacology Source Type: research
itat Sookrung Proviral integration site of Moloney virus-2 (PIM2) is overexpressed in multiple human cancer cells and high level is related to poor prognosis; thus, PIM2 kinase is a rational target of anti-cancer therapeutics. Several chemical inhibitors targeting PIMs/PIM2 or their downstream signaling molecules have been developed for treatment of different cancers. However, their off-target toxicity is common in clinical trials, so they could not be advanced to official approval for clinical application. Here, we produced human single-chain antibody fragments (HuscFvs) to PIM2 by using phage display library, which w...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
Trichodermin, a trichothecene first isolated in Trichoderma species, is a sesquiterpenoid antibiotic that exhibits significant inhibitory activity to the growth of many pathogenic fungi such as Candida albicans, Rhizoctonia solani, and Botrytis cinerea by inhibiting the peptidyl transferase involved in eukaryotic protein synthesis. Trichodermin has also been shown to selectively induce cell apoptosis in several cancer cell lines and thus can act as a potential lead compound for developing anticancer therapeutics. The biosynthetic pathway of trichodermin in Trichoderma has been identified, and most of the involved genes hav...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In this study, a new glycoside hydrolase family 42 (GH42) β-galactosidase-encoding gene, gal42, was cloned from a newly isolated marine bacterium Bacillus sp. BY02 and expressed in Escherichia coli. Structural characterization indicated that the encoding β-galactosidase, Gal42, is a homotrimer in solution, and homology modeling indicated that it retains the zinc binding sites of the Cys cluster. The reaction activity of Gal42 was significantly increased by Zn2+ (229.6%) and other divalent metal ions (Mn2+, Mg2+, and Co2+), while its activity was inhibited by EDTA (53.9%). Meanwhile, the thermo-stability of the Ga...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In this study, mouse model were treated with total body irradiation (TBI) of 0, 4, 8 and 12 Gy, and the intestinal tissues and fecal samples were collected at 6 h, 3.5 d and 7 d post radiation. We found that the intestinal injuries were manifested in a radiation dose-dependent manner. Results from 16S rRNA gene sequencing demonstrated that the diversity of gut microbiota was not significantly affected at the prodromal stage of acute RIII, after 6 h of radiation. At the critical stage of acute RIII, after 3.5 d of radiation, the composition of gut microbiota was correlated with the radiation dose. The Pearson’s correl...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
AbstractPurpose of ReviewWe highlight the unique facets of paediatric nephrology in Africa in terms of the spectrum of kidney diseases, available diagnostic and treatment modalities, kidney healthcare financing options, paediatric nephrology manpower and the contribution of geography and demographics.Recent FindingsPaediatric acute kidney injury in Africa is now commonly due to sepsis rather than gastroenteritis. Steroid-sensitive form of nephrotic syndrome is far more common than was two decades ago.SummaryThe hot arid climate in North Africa and the tropical climate in most of sub-Saharan Africa, and the high rate of con...
Source: Current Pediatrics Reports - Category: Pediatrics Source Type: research
In this study, we aimed to explore protein-protein interactions of YopM with the two components of T3SS, namely LcrV and LcrG. We used bimolecular fluorescence complementation (BiFC) assay and monitored the reassembly of green fluorescence protein in Escherichia coli. As an indicator of the protein-protein interaction, we monitored the in vivo reconstitution of fluorescence by measuring fluorescence intensity and imaging the cells under fluorescence microscope. We showed, for the first time, that YopM interacts with LcrG, but not with LcrV. Here, we propose BiFC assay as a simple method to screen novel interaction partners...
Source: Cell Research - Category: Cytology Authors: Source Type: research
In this study, a chimeric monomeric IDH was successfully constructed by using homo-dimericEscherichia coli IDH as a start point by rational design and site-saturation mutagenesis. The ~67  kDa chimeric enzyme behaved as a monomer in solution, with aKm of 61  μM and akcat of 15  s−1 for isocitrate in the presence of NADP+ and Mn2+. Our result demonstrated that dimeric IDHs have a potential to evolve monomeric ones. The evolution of the IDH family was also discussed.
Source: Protein Science - Category: Biochemistry Authors: Tags: Full ‐Length Paper Source Type: research
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