Viruses, Vol. 13, Pages 1875: A Reverse Transcription Recombinase-Aided Amplification Method for Rapid and Point-of-Care Detection of SARS-CoV-2, including Variants

Viruses, Vol. 13, Pages 1875: A Reverse Transcription Recombinase-Aided Amplification Method for Rapid and Point-of-Care Detection of SARS-CoV-2, including Variants Viruses doi: 10.3390/v13091875 Authors: Fengyun Li Ping He Dongyan Xiong Yakun Lou Qiaosheng Pu Haixia Zhang Huige Zhang Junping Yu The worldwide pandemic caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and its emergence of variants needs rapid and point-of-care testing methods for a broad diagnosis. The regular RT-qPCR is time-consuming and limited in central laboratories, so a broad and large-scale screening requirement calls for rapid and in situ methods. In this regard, a reverse transcription recombinase-aided amplification (RT-RAA) is proposed here for the rapid and point-of-care detection of SARS-CoV-2. A set of highly conserved primers and probes targeting more than 98% of SARS-CoV-2 strains, including currently circulating variants (four variants of concerns (VOCs) and three variants of interest (VOIs)), was used in this study. With the preferred primers, the RT-RAA assay showed a 100% specificity to SARS-CoV-2 from eight other respiratory RNA viruses. Moreover, the assay here is of a high sensitivity and 0.48 copies/μL can be detected within 25 min at a constant temperature (42 °C), which can be realized on portable equipment. Furthermore, the RT-RAA assay demonstrated its high agreement for the detection of SARS-CoV-2 in clinical specimens compared...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research

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In this report, the first case of COVID-19 positive C.auris fungemia detected in Turkey was presented. A 71-year-old male patient with a history of myocardial infarction, diabetes mellitus, donation of a single kidney and lobectomy surgery due to lung cancer was hospitalized in the pandemic thoracic surgery service due to the findings consistent with viral pneumonia on thoracic computed tomography. Favipiravir 2 x 600 mg and intravenous dexamethasone 1 x 6 mg therapy was administered. The patient tested positive for SARS-CoV-2 polymerase chain reaction, and severe involvement of the left lung was detected in the following ...
Source: Mikrobiyoloji Bulteni - Category: Microbiology Authors: Source Type: research
This study aimed to examine the epidemiological characteristics of
Source: The European Journal of Public Health - Category: General Medicine Source Type: research
Vaccination against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and other pathogens with pandemic potential requires safe, protective, inexpensive, and easily accessible vaccines that can be developed and manufactured rapidly at a large scale. DNA vaccines can achieve these criteria, but induction of strong immune responses has often required bulky,...
Source: Proceedings of the National Academy of Sciences - Category: Science Authors: Tags: Engineering, Immunology and Inflammation, Coronavirus (COVID-19) Biological Sciences Source Type: research
Abstract COVID-19 has affected all aspects of life of men and women. However, women are considered to be hit the hardest by the pandemic. The aim of the study was to determine the impact of COVID-19 on women's lifestyle and quality of life (QoL). A questionnaire was developed consisting of sociodemographic data, health condition (presence of chronic condition, BMI, COVID-19 status), selected lifestyle components, stress level (Perceived Stress Scales, PSS-10) and QoL (sense of security/social relationships/mental and physical wellbeing/health care access/financial situation/work satisfaction) in comparison to pre-COVI...
Source: The European Journal of Public Health - Category: General Medicine Source Type: research
The year 2019 has seen an emergence of the novel coronavirus named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causing coronavirus disease of 2019 (COVID-19). Since the onset of the pandemic, biological and interdisciplinary research is being carried out across the world at a rapid pace to beat the pandemic. There is an increased need to comprehensively understand various aspects of the virus from detection to treatment options including drugs and vaccines for effective global management of the disease. In this review, we summarize the salient findings pertaining to SARS-CoV-2 biology, including symptoms, ...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Abstract In December 2019, a novel coronavirus (SARS-CoV-2) appeared in the city of Wuhan, China. Since little was known about the new virus at the time, the initial reaction in many countries, including most countries in Europe, was to follow their existing pandemic influenza response plans. Countries that adopted this approach presumed that case- and contact-based management would be unable to cope with the scale of the outbreak and widespread community transmission would be inevitable. Yet it became increasingly apparent that COVID-19 was not like pandemic influenza, not least because it was caused by a coronavirus...
Source: The European Journal of Public Health - Category: General Medicine Source Type: research
ConclusionsThis study analyses neutralizing antibodies, which should prevent the virus from binding to target cells via spike protein. According to literature, immunological response to vaccine presents a great variability of antibodies level in the study population. However, no significantly correlations were found between the available variables. Preliminary data show the presence of a very intense antibody response after active vaccine immunization both in subjects with previous infection and in the rest of the study population.Key messagesCovid-19 vaccine response in a population at risk.Differences between subjects wi...
Source: The European Journal of Public Health - Category: General Medicine Source Type: research
Muhammad Sohail Zafar The hustle and bustle of the planet Earth have come to a halt thanks to the novel coronavirus. The virus has affected approximately 219 million people globally; taken the lives of 4.55 million patients as of September 2021; and created an ambiance of fear, social distancing, and economic instability. The purpose of this review article is to trace the historical origin and evolution of severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2). The virus is highly contagious with a unique feature of rapid mutations—the scientific research is paving the way for discoveries regarding n...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Review Source Type: research
AbstractThe Covid-19 pandemic crisis caused a serious ‘tectonic' disturbance in all sectors, especially pointed out weaknesses of the healthcare systems. The recently adopted European Green Deal (EGD). It should take into account the Covid-19 pandemic challenge and maintain its primary objectives. The pandemic crisis has shown not only individual hea lth risks, but also global health threats (direct and indirect). The SARS-CoV-2 virus causes an insufficiently known infectious disease. The subsidiary health effects are still countless: mental health, health-economic costs, deadlock in the other diseases treatment, etc...
Source: The European Journal of Public Health - Category: General Medicine Source Type: research
ConclusionsWith the emergence of new variants, it is important to adopt widespread mask wearing to avoid SARS-CoV-2 infection and accelerate RNA negative conversion, until effective levels of vaccination are achieved nationally.Key messagesFace masking was associated with accelerated RNA clearance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).Promoting community mask wearing during COVID-19 pandemic is crucial.
Source: The European Journal of Public Health - Category: General Medicine Source Type: research
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