Autoimmune & inflammatory nmd

Idiopathic inflammatory myopathies (IIM) are a heterogeneous group of progressive autoimmune muscle disorders of unknown etiology. It is postulated that mitochondrial dysfunction and protein aggregation in skeletal muscle lead to myofiber degeneration. However, molecular pathways that contribute to protein aggregation in skeletal muscle are not well defined. Here we have isolated membrane-bound organelles (e.g., nuclei, mitochondria, endoplasmic reticulum (ER), Golgi apparatus and plasma membrane) from muscle biopsies of normal (n=3), IIM (n=10), and mitochondrial myopathy (MM) (n=1) patients for global proteomic analysis using an Orbitrap Fusion mass spectrometer.
Source: Neuromuscular Disorders - Category: Neurology Authors: Source Type: research