The role of PhoP/PhoQ two component system in regulating stress adaptation in Cronobacter sakazakii

In this study, we found the PhoP/PhoQ system in C. sakazakii ATCC BAA-894 enhanced bacterial growth in conditions with low Mg2+, acid pH, and the presence of polymyxin B. Moreover, the ΔphoPQ strain significantly reduced survival following exposure to heat, high osmotic pressure, oxidative or bile salts compared with WT strain. Furthermore, the RNA-seq analysis indicated that 1029 genes were upregulated and 979 genes were downregulated in ΔphoPQ strain. The bacterial secretion system, flagella assembly, beta-Lactam resistance and two-component system pathways were significantly downregulated, while the ABC transporters and microbial metabolism in diverse environments pathways were upregulated. qRT-PCR analysis further confirmed that twelve genes associated with stress tolerance were positively regulated by the PhoP/PhoQ system. Therefore, these findings suggest that the PhoP/PhoQ system is an important regulatory mechanism for C. sakazakii to resist various environmental stress.PMID:34416955 | DOI:10.1016/j.fm.2021.103851
Source: Food Microbiology - Category: Food Science Authors: Source Type: research

Related Links:

With the randomness and immeasurability properties of zero point, the conventional self-supporting film thickness measurement model must ...
Source: Applied Optics - Category: Physics Authors: Source Type: research
The phenomenon of continuous out-of-focus imaging often occurs in high-magnification optical microscopy when observing large-scale targets. ...
Source: Applied Optics - Category: Physics Authors: Source Type: research
To realize the flexible monitoring and online pose correction of the complex assembly of large components, an optical fiber sensor ...
Source: Applied Optics - Category: Physics Authors: Source Type: research
A delay line consisting of a balanced side-coupled integrated spaced sequence of resonators and phase change material VO_2 films is ...
Source: Applied Optics - Category: Physics Authors: Source Type: research
A new, to the best of our knowledge, multi-purpose spectral-polarizing beam splitter (SPBS) design is described that simultaneously ...
Source: Applied Optics - Category: Physics Authors: Source Type: research
Recently, photonic crystal fibers (PCFs) have become of significant interest due to their various applications, especially in the ...
Source: Applied Optics - Category: Physics Authors: Source Type: research
Backlight units (BLUs) with miniLEDs as light sources can help improve display contrast and decrease the thickness of a liquid crystal ...
Source: Applied Optics - Category: Physics Authors: Source Type: research
The acquisition and processing of skylight polarization information forms the cornerstone in modern navigation systems that are developed ...
Source: Applied Optics - Category: Physics Authors: Source Type: research
A synchronous-traveling carrier photodetector (STC-PD) with a thick absorption region and large diameter is presented and investigated for ...
Source: Applied Optics - Category: Physics Authors: Source Type: research
In this paper, we propose a terahertz dual-channel modulator by combining a high electron mobility transistor (HEMT) with a metamaterials ...
Source: Applied Optics - Category: Physics Authors: Source Type: research
More News: Bile | Environmental Health | Food Science | Genetics | Microbiology | Polymyxin B | Study