A synergistic consortium involved in Rac -dichlorprop degradation as revealed by DNA-stable isotope probing and metagenomics analysis
In this study, DNA-stable isotope probing and metagenomics analysis were integrated to reveal the key microbial consortium responsible for Rac-dichlorprop degradation in a Rac-dichlorprop-degrading enrichment. OTU340 (Sphingobium sp.) and OTU348 (Sphingopyxis sp.) were significantly enriched in the 13C-Rac-dichlorprop-labeled heavy DNA fractions. A Rac-dichlorprop degrader, Sphingobium sp. L3, was isolated from the enrichment by traditional enrichment method but with additional supplementation of the antibiotic ciprofloxacin, which was instructed by metagenomics analysis of the associations between Rac-dichlorprop-degraders and antibiotic resistance genes. As revealed by functional profiling of the metagenomes of the heavy DNA, the genes rdpA and sdpA, involved in the initial degradation of the (R)- and (S)-enantiomers of dichlorprop respectively, were mostly taxonomically assigned to Sphingobium species, indicating that Sphingopyxis species might harbor novel dichlorprop degrading genes. In addition, taxonomically diverse bacterial genera such as Dyella, Sphingomonas, Pseudomonas, and Achromobacter were presumed to synergistically cooperate with the key degraders Sphingobium/Sphingopyxis for enhanced degradation of Rac-dichlorprop. Importance Understanding of the key microbial consortium involved in the degradation of the phenoxyalkanoic acid herbicide of Rac-dichlorprop is pivotal for design of synergistic consortia used for enhanced bioremediation of herbicide-contaminated...
"This is the closest we're ever going to get to a linkage between what people are eating or drinking and the health of their liver," said the study's senior author.Medscape Medical News
The Red Sox are in a win-or-go-home situation on Friday in Houston. Follow along all night with commentary from Globe reporters.
Problems with executive function and recall are appearing even in younger cohorts. Effects will have significant repercussions on quality of life, work productivity, and healthcare costs, experts warn.Medscape Medical News
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Series Type : Expression profiling by RT-PCROrganism : Homo sapiensThis SuperSeries is composed of the SubSeries listed below.