Cancers, Vol. 13, Pages 4655: Discordance of PD-L1 Expression at the Protein and RNA Levels in Early Breast Cancer

Cancers, Vol. 13, Pages 4655: Discordance of PD-L1 Expression at the Protein and RNA Levels in Early Breast Cancer Cancers doi: 10.3390/cancers13184655 Authors: Ioannis Zerdes Vaia Karafousia Artur Mezheyeuski Maria Stogiannitsi Raoul Kuiper Pablo Moreno Ruiz George Rassidakis Jonas Bergh Thomas Hatschek Theodoros Foukakis Alexios Matikas We aimed to assess if the discrepant prognostic information between Programmed Death Ligand 1 (PD-L1) protein versus mRNA expression in early breast cancer (BC) could be attributed to heterogeneity in its expression. PD-L1 protein and mRNA expression in BC tissue microarrays from two clinical patient cohorts were evaluated (105 patients; cohort 1: untreated; cohort 2: neoadjuvant chemotherapy-treated). Immunohistochemistry (IHC) with SP142, SP263 was performed. PD-L1 mRNA was evaluated using bulk gene expression and RNA-FISH RNAscope®, the latter scored in a semi-quantitative manner and combined with immunofluorescence (IF) staining for the simultaneous detection of PD-L1 protein expression. PD-L1 expression was assessed in cores as a whole and in two regions of interest (ROI) from the same core. The cell origin of PD-L1 expression was evaluated using multiplex fluorescent IHC. IHC PD-L1 expression between SP142 and SP263 was concordant in 86.7% of cores (p < 0.001). PD-L1 IF/IHC was weakly correlated with spatial mRNA expression (concordance 54.6–71.2%). PD-L1 was mostly expressed by lymphocytes ...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research

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Technol Cancer Res Treat. 2021 Jan-Dec;20:15330338211035037. doi: 10.1177/15330338211035037.ABSTRACTBACKGROUND: Oncotype Dx (ODx) is a genomic assay which estimates the risk of distant recurrence and predicts adjuvant chemotherapy benefit in early stage breast cancer patients. Most ODx data is derived from excisional specimens.AIM: We assess the utility of ODx on core needle biopsies (CNB) and measure its impact on neoadjuvant treatment decisions, particularly in patients with clinically complicated situations.METHODS: Consecutive ODx results on breast CNBs with invasive carcinoma from 2012-2020 at 3 tertiary care hospital...
Source: Technology in Cancer Research and Treatment - Category: Cancer & Oncology Authors: Source Type: research
ConclusionSix cycles of TEC with prophylactic G-CSF is an effective and tolerable neoadjuvant regime for HER2-negative breast cancers. Hematological toxicities were the most common toxicities. Although many patients required dose reduction, all patients completed treatment and there was no observed cardiotoxicity.
Source: Advances in Therapy - Category: Drugs & Pharmacology Source Type: research
Clin Breast Cancer. 2021 Sep 29:S1526-8209(21)00288-3. doi: 10.1016/j.clbc.2021.09.010. Online ahead of print.NO ABSTRACTPMID:34688554 | DOI:10.1016/j.clbc.2021.09.010
Source: Clinical Breast Cancer - Category: Cancer & Oncology Authors: Source Type: research
This study aims to observe and analyze the effect of diffusion weighted magnetic resonance imaging (MRI) on the patients with locally advanced breast cancer undergoing neoadjuvant chemotherapy.
Source: BMC Medical Imaging - Category: Radiology Authors: Tags: Research Source Type: research
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Source: Journal of the American College of Surgeons - Category: Surgery Authors: Tags: Breast Source Type: research
CONCLUSIONS: Our results highlight the variable expression of senescence- and apoptosis-associated markers in breast tumors (despite the shared somatic genetic background) and invites a large-scale assessment of both senescence and apoptosis in breast cancer tissue in vivo and their contribution to the pathological response and overall survival.PMID:34677245 | PMC:PMC8535022 | DOI:10.3390/curroncol28050327
Source: Current Oncology - Category: Cancer & Oncology Authors: Source Type: research
We report on a 52-year-old woman with breast cancer admitted in our institution for onycholysis. Because of the stage and histology of breast cancer, neoadjuvant chemotherapy was initiated. The patient received 8 cycles of Taxotere and Adriamycin (AT), and she underwent a modified radical mastectomy. Three months later, the patient developed evidence of onycholysis, involving all the fingernails. We observed the following changes in nails of all the digits in both hands: onycholysis, dystrophy, oedema, and exudate. Nail scraping and purulent discharge were collected and cultured on Sabouraud medium. Physical features of th...
Source: Case Reports in Oncology - Category: Cancer & Oncology Source Type: research
Condition:   Triple Negative Breast Cancer (TNBC) Interventions:   Drug: Camrelizumab;   Drug: Doxorubicin;   Drug: Cyclophosphamide;   Drug: Docetaxel Sponsor:   Aiping Shi Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Oncol Lett. 2021 Dec;22(6):819. doi: 10.3892/ol.2021.13080. Epub 2021 Oct 5.ABSTRACTTriple-negative breast cancer (TNBC) is characterized by aggressive clinicopathological features and is associated with a poor prognosis. Identifying patients that are non-responsive to chemotherapy remains a critical goal for effective personalized therapies. In the present study, the predictive value of exosomal microRNAs (miRNAs) was investigated in patients with TNBC. Exosomes were isolated from patients with TNBC undergoing neoadjuvant chemotherapy. Microarray-based miRNA profiles were compared between patients with pathological comple...
Source: Oncology Letters - Category: Cancer & Oncology Authors: Source Type: research
Ann Surg Oncol. 2021 Oct 20. doi: 10.1245/s10434-021-10830-4. Online ahead of print.NO ABSTRACTPMID:34671883 | DOI:10.1245/s10434-021-10830-4
Source: Ann Oncol - Category: Cancer & Oncology Authors: Source Type: research
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