Rift Valley fever virus 78kDa envelope protein attenuates virus replication in macrophage-derived cell lines and viral virulence in mice

by Kaori Terasaki, Birte Kalveram, Kendra N. Johnson, Terry Juelich, Jennifer K. Smith, Lihong Zhang, Alexander N. Freiberg, Shinji Makino Rift Valley fever virus (RVFV) is a mosquito-borne bunyavirus with a wide host range including ruminants and humans. RVFV outbreaks have had devastating effects on public health and the livestock industry in African countries. However, there is no approved RVFV vaccine for human use in non-endemic countries and no FDA-approved antiviral drug for RVFV treatment. The RVFV 78kDa protein (P78), which is a membrane glycoprotein, plays a role in virus dissemination in the mosquito host, but its biological role in mammalian hosts remains unknown. We generated an attenuated RVFV MP-12 strain-derived P78-High virus and a virulent ZH501 strain-derived ZH501-P78-High virus, both of which expressed a higher level of P78 and carried higher levels of P78 in the virion compared to their parental viruses. We also generated another MP-12-derived mutant virus (P78-KO virus) that does not express P78. MP -12 and P78-KO virus replicated to similar levels in fibroblast cell lines and Huh7 cells, while P78-High virus replicated better than MP-12 in Vero E6 cells, fibroblast cell lines, and Huh7 cells. Notably, P78-High virus and P78-KO virus replicated less efficiently and more efficiently, respectivel y, than MP-12 in macrophage cell lines. ZH501-P78-High virus also replicated poorly in macrophage cell lines. Our data further suggest that inefficient binding ...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research

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Contributors : Ana I Nu ñez ; Anna Esteve-Codina ; Jèssica Gómez-Garrido ; Marc Dabad ; Marco Brustolin ; Sandra Talavera ; Miguel Berdugo ; Tyler Alioto ; Albert Bensaid ; Núria BusquetsSeries Type : Expression profiling by high throughput sequencingOrganism : Culex pipiensRift valley fever (RVF) is an emerging zoonotic disease and it is caused by Rift valley fever phlebovirus (RVFV). This virus is commonly transmitted in endemic areas between wild ruminants and mosquitoes, mainly by mosquitoes of Culex and Aedes genus. Starting from 2000, several outbreaks have been reported outside the Africa...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Culex pipiens Source Type: research
Comparative thermo-stability of two Rift Valley fever virus vaccine candidate CL13T with a recombinant arMP-12ΔNSm21/384. Bioinformation. 2020;16(7):547-554 Authors: Daouam S, Boumart Z, Elarkam A, Hamdi J, Tadlaoui KO, Ennaji MM, Harraka M Abstract Rift Valley fever (RVF) is a zoonotic, viral disease, transmitted by mosquitoes, characterized by high mortality rates in young animals. RVF is an endemic and enzootic disease in the Arabian Peninsula and Africa, causing public health and economic instability. Therefore, it is important to develop vaccines to minimize outbreaks and combat the disease...
Source: Bioinformation - Category: Bioinformatics Authors: Tags: Bioinformation Source Type: research
In this study, we exposed apically well-differentiated human NECs cultured at the ALI to the related flaviviruses ZIKV, JEV, WNV, and Usutu virus (USUV). We selected these viruses due to the recent increasing evidences of potential threat to humans (Cadar et al., 2017; Simonin et al., 2018). We show that NECs are particularly susceptible to JEV and WNV infection and to other flaviviruses included in this study. Infection with each virus led to shedding of infectious virus particles through the apical and basolateral surfaces and triggered host mechanisms at the level of inflammatory and antiviral mediators. Given...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
This report summarizes the lectures held at the meeting and highlights advances in the field.
Source: Antiviral Therapy - Category: Virology Source Type: research
This report summarizes the lectures held at the meeting and highlights advances in the field. PMID: 30597184 [PubMed - as supplied by publisher]
Source: Antiviral Research - Category: Virology Authors: Tags: Antiviral Res Source Type: research
Publication date: April 2018Source: Current Opinion in Virology, Volume 29Author(s): Baptiste Dungu, Baratang A Lubisi, Tetsuro IkegamiRift Valley fever (RVF) is a zoonotic mosquito-borne bunyaviral disease associated with high abortion rates, neonatal deaths, and fetal malformations in ruminants, and mild to severe disease in humans. Outbreaks of RVF cause huge economic losses and public health impacts in endemic countries in Africa and the Arabian Peninsula. A proper vaccination strategy is important for preventing or minimizing outbreaks. Vaccination against RVF is not practiced in many countries, however, due to absenc...
Source: Current Opinion in Virology - Category: Virology Source Type: research
In this study, we aimed to strengthen the attenuation profile of the MP-12 vaccine strain via the introduction of 584 silent mutations. To minimize the impact on protective efficacy, codon usage and codon pair bias were not de-optimized. The resulting rMP12-GM50 strain showed 100% protective efficacy with a single intramuscular dose, raising a 1:853 mean titer of plaque reduction neutralization test. Moreover, outbred mice infected with one of three pathogenic reassortant ZH501 strains, which encoded rMP12-GM50 L-, M-, or S-segments, showed 90%, 50%, or 30% survival, respectively. These results indicate that attenuation of...
Source: Vaccine - Category: Allergy & Immunology Authors: Tags: Vaccine Source Type: research
Summary Rift Valley fever virus (RVFV) causes consistently severe outbreaks with high public health impacts and economic losses in livestock in many African countries and has also been introduced to Saudi Arabia and Yemen. Egypt with its four large outbreaks in the last 40 years represents the northernmost endemic area of RVFV. The purpose of this study was to provide an insight into the current anti‐RVFV antibody status in immunized as well as non‐immunized dairy cattle from the Nile Delta of Egypt. During 2013–2015, a total of 4,167 dairy cattle from four governorates including Dakahlia, Damietta, Gharbia ...
Source: Transboundary and Emerging Diseases - Category: Veterinary Research Authors: Tags: ORIGINAL ARTICLE Source Type: research
Conclusion/SignificanceReactive vaccination can be effective in mitigating the impacts of RVF outbreaks but practically, it is not always possible to have this measure implemented satisfactorily due to the rapid onset and evolution of RVF epidemics. This analysis demonstrates that both periodic and reactive vaccination ought to be used strategically to effectively control the disease.
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
Publication date: Available online 20 January 2016 Source:Antiviral Research Author(s): Alan Baer, Nazly Shafagati, Ashwini Benedict, Tatiana Ammosova, Andrey Ivanov, Ramin M. Hakami, Kaori Terasaki, Shinji Makino, Sergei Nekhai, Kylene Kehn-Hall Rift Valley fever virus (RVFV), genus Phlebovirus family Bunyaviridae, is an arthropod-borne virus endemic throughout sub-Saharan Africa. Recent outbreaks have resulted in cyclic epidemics with an increasing geographic footprint, devastating both livestock and human populations. Despite being recognized as an emerging threat, relatively little is known about the virule...
Source: Antiviral Therapy - Category: Virology Source Type: research
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