Deficiency of Pol η in < i > Saccharomyces cerevisiae < /i > reveals the impact of transcription on damage-induced cohesion

by Pei-Shang Wu, Jan Grosser, Donald P. Cameron, Laura Baranello, Lena Str öm

The structural maintenance of chromosome (SMC) complex cohesin mediates sister chromatid cohesion established during replication, and damage-induced cohesion formed in response to DSBs post-replication. The translesion synthesis polymerase Polη is required for damage-induced cohesion through a hi therto unknown mechanism. Since Polη is functionally associated with transcription, and transcription triggersde novo cohesion inSchizosaccharomyces pombe, we hypothesized that transcription facilitates damage-induced cohesion inSaccharomyces cerevisiae. Here, we show dysregulated transcriptional profiles in the Pol η null mutant (rad30 Δ), where genes involved in chromatin assembly and positive transcription regulation were downregulated. In addition, chromatin association of RNA polymerase II was reduced at promoters and coding regions inrad30 Δ compared to WT cells, while occupancy of the H2A.Z variant (Htz1) at promoters was increased inrad30 Δ cells. Perturbing histone exchange at promoters inactivated damage-induced cohesion, similarly to deletion of theRAD30 gene. Conversely, altering regulation of transcription elongation suppressed the deficient damage-induced cohesion inrad30 Δ cells. Furthermore, transcription inhibition negatively affected formation of damage-induced cohesion. These results indicate that the transcriptional deregulation of the Pol η null mutant is connected with its re...

Source: PLoS Genetics - September 9, 2021 Category: Genetics & Stem Cells Authors: Pei-Shang Wu Source Type: research