Dual photonic bandgap hollow sphere colloidal photonic crystals for real-time fluorescence enhancement in living cells

Biosens Bioelectron. 2021 Aug 27;194:113577. doi: 10.1016/j.bios.2021.113577. Online ahead of print.ABSTRACTTo overcome the problems of refractive index matching and increased disorder when working with traditional heterostructure colloidal photonic crystals (CPCs) with dual or multiple photonic bandgaps (PBGs) for fluorescence enhancement in water, we propose the use of a chemical heterostructure in hollow sphere CPCs (HSCPCs). A partial chemical modification of the HSCPC creates a large contrast in wettability to induce the heterostructure, while the hollow spheres increase the refractive index difference when used in aqueous environment. With the platform, fluorescence enhancement reaches around 160 times in solution, and 72 times (signal-to-background ratio ~7 times) in cells during proof-of-concept live cardiomyocyte contractility experiments. Such photonic platform can be further exploited for chemical sensing, bioassays, and environmental monitoring. Moreover, the introduction of chemical heterostructures provides new design principles for functionalized photonic devices.PMID:34481238 | DOI:10.1016/j.bios.2021.113577
Source: Biosensors and Bioelectronics - Category: Biotechnology Authors: Source Type: research