Nanopore detects γ-radiation inhibited HIV-1 protease activity

Biosens Bioelectron. 2021 Aug 30;194:113602. doi: 10.1016/j.bios.2021.113602. Online ahead of print.ABSTRACTInhibition of HIV-1 protease (PR) activity is realized by exposure to 60Co γ-radiation. The radiation effects on enzyme kinetics of HIV-1 PR are subsequently monitored using nanopore sensor. Substantial loss of proteolytic efficiency towards GagPol polypeptide is observed due to the radiation treatment. Results shows ~50% of GagPol polypeptide was not involved in HIV-1 PR proteolysis by exposure to ultra-low intensity of γ-radiation (0.1K Gy), and the values reach to over 90% with high γ-ray treatment. Besides, the inactivation effect is also verified in blood samples which contain the virus protease. Our finding provides the potential benefits of γ-radiation to inactivate viral proteinic function, and might be a complementary to the designation of HIV-1 PR inhibitors.PMID:34481241 | DOI:10.1016/j.bios.2021.113602
Source: Biosensors and Bioelectronics - Category: Biotechnology Authors: Source Type: research