Cancers, Vol. 13, Pages 4473: Necroptosis in Esophageal Squamous Cell Carcinoma: An Independent Prognostic Factor and Its Correlation with Tumor-Infiltrating Lymphocytes

Cancers, Vol. 13, Pages 4473: Necroptosis in Esophageal Squamous Cell Carcinoma: An Independent Prognostic Factor and Its Correlation with Tumor-Infiltrating Lymphocytes Cancers doi: 10.3390/cancers13174473 Authors: Takuro Yamauchi Fumiyoshi Fujishima Masatoshi Hashimoto Junichi Tsunokake Ryujiro Akaishi Yusuke Gokon Shunsuke Ueki Yohei Ozawa Toshiaki Fukutomi Hiroshi Okamoto Chiaki Sato Yusuke Taniyama Tomohiro Nakamura Naoki Nakaya Takashi Kamei Hironobu Sasano Necroptosis is a pivotal process in cancer biology; however, the clinical significance of necroptosis in esophageal squamous cell carcinoma (ESCC) has remained unknown. Therefore, in this study, we aimed to verify the potential involvement of necroptosis in the clinical outcome, chemotherapeutic resistance, and tumor microenvironment of ESCC. Mixed lineage kinase domain-like protein (MLKL) and phosphorylated MLKL (pMLKL) were immunohistochemically examined in 88 surgically resected specimens following neoadjuvant chemotherapy (NAC) and 53 pre-therapeutic biopsy specimens, respectively. Tumor-infiltrating lymphocytes (TILs) were also evaluated by immunolocalizing CD3, CD8, and forkhead box protein 3 (FOXP3) in the residual tumors after NAC. High pMLKL status in the post-NAC resected specimens was significantly correlated with worse prognosis in ESCC patients. Multivariate analysis demonstrated that a high pMLKL status was an independent prognostic factor. In pre-NAC biopsy specime...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research