Proline ‐rich Extensin‐like Receptor Kinases PERK5 and PERK12 are involved in Pollen Tube Growth

AbstractProline-richextensin-likereceptorkinases (PERKs) belong to the hydroxyproline-rich glycoprotein (HRGP) superfamily known to be involved in many plant developmental processes. Here, we characterized two pollen-expressedPERKs fromArabidopsis thaliana,PERK5 andPERK12. Pollen tube growth was impaired in single and doubleperk5-1 perk12-1 loss of function mutants, with an impact on seed production. When the segregation was analysed, a male gametophytic defect was found, indicating thatperk5-1 andperk12-1 mutants carry deficient pollen transmission. Furthermore,perk5-1 perk12-1 displayed an excessive accumulation of pectins and cellulose at the cell wall of the pollen tubes. Our results indicate thatPERK5 andPERK12 are necessary for proper pollen tube growth, highlighting their role in cell wall assembly and reactive oxygen species homeostasis.

Source: FEBS Letters - August 26, 2021 Category: Biochemistry Authors: Cecilia Borassi, Ana R. Sede, Mart ín A. Mecchia, Silvina Mangano, Eliana Marzol, Silvina P. Denita‐Juarez, Juan D. Salgado Salter, Silvia M. Velasquez, Jorge P. Muschietti, José M. Estevez Tags: RESEARCH LETTER Source Type: research