CRISPR/Cas advancement in molecular diagnostics and signal readout approaches

We present here the major three Cas-effectors (Cas9, Cas12 and Cas13) and their significance in various detection assays. The CRISPR/Cas detection strategies based on their target hybridization, cleavage activity, sensor capabilities and signal readout methods are discussed.

Source: Journal of Molecular Diagnostics - August 25, 2021 Category: Pathology Authors: Md Zohaib Ahmed, Purav Badani, Raj Reddy, Geetesh Mishra Tags: Review Source Type: research