Homocysteine: validation and comparison of two methods using samples from patients with pulmonary hypertension

Conclusion: The validated HPLC method showed good performance for quantification of plasma homocysteine levels, while S-Ec method provided results for homocysteine comparable with those obtained by the validated method; therefore, this methodology is a potential alternative of automated method for clinical laboratories. Introdução e objetivo: A determinação dos níveis plasmáticos de homocisteína tem sido relatada como um marcador de risco de interesse em doenças graves que cursam com lesões endoteliais, estando associada ao desenvolvimento ou à progressão de lesões ateroscleróticas e formação de trombos. Os objetivos do presente estudo compreenderam validar o método de dosagem de homocisteína plasmática por cromatografia líquida de alta eficiência (CLAE) com detecção fluorimétrica, analisar amostras de pacientes com hipertensão pulmonar e comparar os resultados obtidos por CLAE com aqueles obtidos com a metodologia espectrofotométrica enzimática cíclica (E-Ec). Materiais e métodos: Os parâmetros de validação linearidade, efeito de matriz, precisão, exatidão, limites de detecção e quantificação, além de robustez do método foram avaliados visando demonstrar que este está apropriado para o uso pretendido. ...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - Category: Pathology Source Type: research

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Publication date: Available online 10 November 2019Source: Revista Clínica Española (English Edition)Author(s): G. Serralta San Martín, J. Canora Lebrato, en representación del Grupo de Trabajode Ecografía Clínica y del Grupo de Trabajo de Enfermedades Autoinmunes Sistémicas de la Sociedad Españ ola de Medicina InternaAbstractClinical ultrasonography should be considered a complementary examination that can change clinical practice, as well as a tool to add to the medical history.Systemic autoimmune diseases (SAD) can involve numerous structures and organs. Ultrasonog...
Source: Revista Clinica Espanola - Category: Internal Medicine Source Type: research
We present the case report of a 26-year-old woman who became pregnant following the successful treatment of severe CTEPH by utilizing BPA. We conclude that patients undergoing effective BPA for CTEPH can consider becoming pregnant if followed closely by a multidisciplinary team, including experts in thrombosis, pulmonary hypertension and obstetrics.
Source: Canadian Journal of Cardiology - Category: Cardiology Source Type: research
Although a number of risk factors for chronic thromboembolic pulmonary hypertension (CTEPH) have been reported, the exact prevalence is controversial and varies between published cohorts. The aim of the present study was to investigate the prevalence of risk factors in operable CTEPH patients with special emphasis on thyroid disease and function.
Source: Thrombosis Research - Category: Hematology Authors: Tags: Full Length Article Source Type: research
Surviving an embolism exposes patients to potential long-term complications, such as altered quality of life, persistent dyspnea, impaired exercise capacity or pulmonary hypertension. The common objective factor in most of these situations is the presence of residual pulmonary vascular obstruction (RPVO). Planar ventilation/perfusion scintigraphy (V/Q lung scan) is the gold standard for assessing RPVO, which occurs in 46 to 66% of patients at 3  months and persists in 25 to 29% of patients a year after acute PE.
Source: Thrombosis Research - Category: Hematology Authors: Tags: Review Article Source Type: research
ConclusionHSS is a rare type of vasculitis, which can present with arterial, venous and/or intracardiac thrombosis even without other systemic manifestations. HSS can present without venous involvement. HSS require aggressive immune suppressive treatment, however anticoagulation and surgery can be attempted when indicated.
Source: The Egyptian Rheumatologist - Category: Rheumatology Source Type: research
At altitudes>2,500 meters, acute mountain sickness is usually self-limiting, presenting with hypoxia and pulmonary edema.1 Chronic mountain sickness results in pulmonary hypertension and the development of congestive cardiac failure.1,2 Hypoxemia is associated with signs of peripheral cyanosis. Blue toe syndrome is a common presentation of peripheral ischemia due to occlusion of small caliber peripheral vessels. The differential diagnosis includes atheroembolism, thromboembolism, and vasospastic conditions.
Source: The American Journal of Medicine - Category: General Medicine Authors: Tags: DIAGNOSTIC DILEMMA Source Type: research
Abstract BACKGROUND: Although cardiac troponin and natriuretic peptide have been shown to decrease after balloon pulmonary angioplasty (BPA) with improved right ventricular afterload in chronic thromboembolic pulmonary hypertension (CTEPH), biomarkers to evaluate the effects of BPA independently of heart failure status remain to be developed. METHODS: In 39 consecutive CTEPH patients including 31 who underwent BPA, we measured plasma levels of cyclophilin A (CyPA), which we demonstrated is secreted from pulmonary vascular smooth muscle cells in response to mechanical stretch and hypoxia. RESULTS: CyPA le...
Source: Journal of Cardiology - Category: Cardiology Authors: Tags: J Cardiol Source Type: research
CONCLUSIONS: In HIMF-induced PH, HMGB1-RAGE signaling is pivotal for mediating EC-smooth muscle cell cross talk. The humanized mouse data further support clinical implications for the HIMF/HMGB1 signaling axis and indicate that hResistin and its downstream pathway may constitute targets for the development of novel anti-PH therapeutics in humans. PMID: 31597444 [PubMed - as supplied by publisher]
Source: Arteriosclerosis, Thrombosis and Vascular Biology - Category: Cardiology Authors: Tags: Arterioscler Thromb Vasc Biol Source Type: research
Etiology of Exercise-Induced Pulmonary Hypertension Can Be Differentiated by Echocardiography - Insight From Patients With Chronic Pulmonary Thromboembolism With Normal Resting Hemodynamics by Balloon Pulmonary Angioplasty. Circ J. 2019 Oct 02;: Authors: Goda A, Takeuchi K, Kikuchi H, Finger M, Inami T, Sakata K, Soejima K, Satoh T Abstract BACKGROUND: Exercise-induced pulmonary hypertension (PH) is often seen in chronic thromboembolic PH (CTEPH) patients with normalized resting hemodynamics, but it is difficult to differentiate precapillary PH as pulmonary vascular dysfunction and post-capillary PH...
Source: Circulation Journal - Category: Cardiology Authors: Tags: Circ J Source Type: research
Abstract BACKGROUND: Chronic thromboembolic pulmonary hypertension (CTEPH) is an uncommon complication of acute pulmonary emboli necessitating lifelong anticoagulation. Despite this, little data exists on the safety and efficacy of Vitamin K Antagonists (VKAs) in CTEPH and none for Direct Oral Anticoagulants (DOACs). OBJECTIVES: To evaluate outcomes and complication rates in CTEPH following Pulmonary Endarterectomy (PEA) for individuals receiving VKAs or DOACs. METHODS: Consecutive CTEPH patients undergoing PEA between 2007 and 2018 were included in a retrospective analysis. Post-operative outcomes, recu...
Source: Thrombosis and Haemostasis - Category: Hematology Authors: Tags: J Thromb Haemost Source Type: research
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