O-074 No methylome differences observed in IVF children born after embryo culture in different culture media

AbstractStudy questionDoes human embryo culture in different IVF culture media lead to DNA methylation alterations in IVF offspring?Summary answerGenome-wide analyses identified no significant DNA methylation differences between culture medium groups in IVF children (neonates or 9-year olds) from two culture media studies.What is known alreadyDuring in vitro fertilisation (IVF) treatments, embryos undergo preimplantation development in an artificial environment, while concurrently undergoing epigenetic reprogramming. Adversity during this period, such as peri-conception calorie restriction, has been linked to persistent DNA methylation aberrations and increased risk of cardiometabolic disease. Early environmental adversity is suspected in IVF offspring as they are born with lower birthweights and show increased risk of cardiometabolic dysfunction in adulthood as compared to their naturally-conceived counterparts. This is further supported by the observation from two culture media trials (MEDIUM0 and MEDIUM1) that embryo culture in different culture media leads to differences in birthweight.Study design, size, durationWe recruited singleton offspring from two IVF culture media trials. The MEDIUM0 study, a pseudo-randomized trial comparing G3 (Vitrolife) and K-SICM (Cook), was conducted from 2003-2006. At the 9-year follow-up, saliva was collected (cohort-A). The MEDIUM1 study, a multi-center randomized controlled trial comparing G5 (Vitrolife) and HTF (Lonza), was conducted fr...
Source: Human Reproduction - Category: Reproduction Medicine Source Type: research

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J Obstet Gynaecol. 2021 Sep 29:1-7. doi: 10.1080/01443615.2021.1959536. Online ahead of print.ABSTRACTWe performed a retrospective cohort study, namely "Surgery and ART for Endometriomas" (SAFE) trial (Clinical Trial ID: NCT03717870), including women who underwent laparoscopic cystectomy for endometrioma before first IVF and compared their reproductive outcomes with the ones of women without endometriosis and with unexplained infertility, tubal factor or male factor infertility. We found that women who underwent previous laparoscopic cystectomy for endometrioma had higher FSH and LH levels between the 2nd and 5th...
Source: Journal of Obstetrics and Gynaecology - Category: OBGYN Authors: Source Type: research
In this study, whole-exome sequencing was performed to identify the causative genes in ten unrelated infertile patients with PCD: seven males and three females. Notably, homozygous frameshift mutations in MCIDAS (c.186dupT, p.Pro63Serfs*22) and CCNO (c.262_263insGGCCC, p.Gln88Argfs*8) were identified in one male and one female participant from two unrelated consanguineous families. Haematoxylin-eosin staining/scanning electron microscopy revealed abnormal MCCs in the mutated EDs/oviducts. Furthermore, transmission electron microscopy revealed significantly reduced BBs. Immunofluorescence staining showed the absence of MCID...
Source: Clinical Genetics - Category: Genetics & Stem Cells Authors: Source Type: research
[Cameroon Tribune] The three babies were conceived via In-Vitro Fertilisation (IVF) and delivered last week at the reproductive hospital in Yaounde.
Source: AllAfrica News: Pregnancy and Childbirth - Category: OBGYN Source Type: news
CONCLUSIONS: Higher maternal BMI is associated with faster early preimplantation development. In couples with female or combined factor subfertility, a higher BMI is associated with a lower implantation potential as predicted by the KIDScore. Likely due to power issues, we did not observe an impact on clinical treatment outcomes. However, an effect of faster preimplantation development on post-implantation development is conceivable, especially since the impact of maternal BMI on pregnancy outcomes has been widely demonstrated.PMID:34537064 | DOI:10.1186/s12958-021-00822-0
Source: Reproductive Biology - Category: Reproduction Medicine Authors: Source Type: research
CONCLUSIONS: MiR-30d-5p and SOCS1 may be potential biomarkers for endometrial receptivity. Changes in pinopode development and abnormal expression of miR-30d-5p, SOCS1 and LIF pathway in the endometrium could be the reasons for implantation failure.PMID:34496883 | DOI:10.1186/s12958-021-00820-2
Source: Reproductive Biology - Category: Reproduction Medicine Authors: Source Type: research
CONCLUSIONS: We are uncertain whether intravenous atosiban improves pregnancy outcomes for women undergoing assisted reproductive technology. This conclusion is based on currently available data from seven RCTs, which provided very low- to low-certainty evidence across studies. We could draw no clear conclusions about subcutaneous barusiban, based on limited data from one RCT. Further large well-designed RCTs reporting on live births and adverse clinical outcomes are still required to clarify the exact role of atosiban and barusiban before ET. Oral nolasiban appears to improve clinical pregnancy rate but not live birth rat...
Source: Cochrane Database of Systematic Reviews - Category: General Medicine Authors: Source Type: research
AbstractStudy questionIs there a difference in treatment outcome between gonadotrophin releasing hormone (GnRH) pump or hMG for women with Type 1 anovulation undergoing ovulation induction?Summary answerTreatment with GnRH was more efficient compared to hMG, with fewer number of cycles to pregnancy, fewer days of stimulation and fewer cycle cancellations.What is known alreadyWhilst there is a lot of information on ovulation induction in WHO type II anovulation (PCOS), type 1 anovulation is under-represented in research. WHO type 1 anovulation is characterised by low pituitary gonadotrophins and oestradiol. Treatment option...
Source: Human Reproduction - Category: Reproduction Medicine Source Type: research
This study is part of a completed multi-centre randomised placebo-controlled trial (IMPLANT 1 – NCT02310802) in IVF patients (n = 247) carried out in 2015. Our study retrospectively assessed a sub-set of patients with good quality TVUS recordings to evaluate their mechanical uterine motion that were randomised to receive either nolasiban 900mg (n = 39) or placebo (n = 42).Participants/materials, setting, methodsSubjects were
Source: Human Reproduction - Category: Reproduction Medicine Source Type: research
AbstractStudy questionAre obstetric/perinatal outcomes different in singleton pregnancies following blastocyst-stage embryo transfer when compared to cleavage-stage embryo transfer and have results changed over time?Summary answerPregnancies following blastocyst are consistently associated with higher risk of large for gestational age and lower risk of small for gestational age babiesWhat is known alreadyExtended embryo culture to blastocyst-stage is widely used to select best embryos in in vitro fertilisation (IVF) cycles to improve pregnancy rates. Transfer of blastocyst-stage embryos is increasing with this being the de...
Source: Human Reproduction - Category: Reproduction Medicine Source Type: research
AbstractStudy questionWhat are a woman ’s contraceptive needs after successful in vitro fertilisation (IVF) pregnancy? and how should services respond to help prevent unintended pregnancies?Summary answerWomen who have IVF pregnancies require tailored, postnatal contraception counselling. Services must provide evidence-based information about the risks of spontaneous conception to engage them effectively.What is known alreadyWomen undergoing IVF are an increasingly heterogeneous group with a wide range of causative subfertility factors. Furthermore, increasingly, women are accessing treatment primarily for reasons ot...
Source: Human Reproduction - Category: Reproduction Medicine Source Type: research
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