PVA based nanofiber containing cellulose modified with graphitic carbon nitride/nettles/trachyspermum accelerates wound healing

This study aimed to fabricate and study bacterial cellulose/graphitic carbon nitride/nettles/trachyspermum nanocomposite by immersion and PVA/BC/g-C3N4/nettles/trachyspermum nanofiber by electrospinning method as a wound dressing. The g-C3N4 and g-C3N4 solution were synthesized and then were characterized using FTIR, XRD, Zeta Potential, and SEM analyzes. Also, the antibacterial properties of the synthesized materials were proved by gram-positive and gram-negative bacteria using the MIC method. Besides, the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts were evaluated using MTT and scratch assays and showed that the BC/PVA/g-C3N4/nettles/trachyspermum composite not only had no toxic effect on cells but also contributed to cell survival, cell migration, and proliferation has done. To evaluate the mechanical properties, a tensile strength test was performed on PVA/BC/g-C3N4/nettles/trachyspermum nanofibers, and the results showed good strength of the nanocomposite. In addition,in vivo assay, the produced nanofibers were used to evaluate wound healing, and the results showed that these nanofibers were able to accelerate the wound healing process so that after 14  days, the wound healing percentage showed 95%. Therefore, this study shows that PVA/BC/g-C3N4/nettles /trachyspermum nanofibers effectively inhibit bacterial growth and accelerate wound healing.This article is protected by copyright. All rights reserved.
Source: Biotechnology Progress - Category: Biotechnology Authors: Tags: RESEARCH ARTICLE Source Type: research