Genes, Vol. 12, Pages 1191: Severity of Idiopathic Scoliosis Is Associated with Differential Methylation: An Epigenome-Wide Association Study of Monozygotic Twins with Idiopathic Scoliosis
Genes, Vol. 12, Pages 1191: Severity of Idiopathic Scoliosis Is Associated with Differential Methylation: An Epigenome-Wide Association Study of Monozygotic Twins with Idiopathic Scoliosis
Genes doi: 10.3390/genes12081191
Authors:
Patrick M. Carry
Elizabeth A. Terhune
George D. Trahan
Lauren A. Vanderlinden
Cambria I. Wethey
Parvaneh Ebrahimi
Fiona McGuigan
Kristina Åkesson
Nancy Hadley-Miller
Epigenetic mechanisms may contribute to idiopathic scoliosis (IS). We identified 8 monozygotic twin pairs with IS, 6 discordant (Cobb angle difference >10°) and 2 concordant (Cobb angle difference ≤2°). Genome-wide methylation in blood was measured with the Infinium HumanMethylation EPIC Beadchip. We tested for differences in methylation and methylation variability between discordant twins and tested the association between methylation and curve severity in all twins. Differentially methylated region (DMR) analyses identified gene promoter regions. Methylation at cg12959265 (chr. 7 DPY19L1) was less variable in cases (false discovery rate (FDR) = 0.0791). We identified four probes (false discovery rate, FDR < 0.10); cg02477677 (chr. 17, RARA gene), cg12922161 (chr. 2 LOC150622 gene), cg08826461 (chr. 2), and cg16382077 (chr. 7) associated with curve severity. We identified 57 DMRs where hyper- or hypo-methylation was consistent across the region and 28 DMRs with a consistent association with curve severity. Among DMRs, 21 were correlate...
Source: Genes - Category: Genetics & Stem Cells Authors: Patrick M. Carry Elizabeth A. Terhune George D. Trahan Lauren A. Vanderlinden Cambria I. Wethey Parvaneh Ebrahimi Fiona McGuigan Kristina Åkesson Nancy Hadley-Miller Tags: Article Source Type: research