Viruses, Vol. 13, Pages 1487: Localization of SARS-CoV-2 Capping Enzymes Revealed by an Antibody against the nsp10 Subunit

Viruses, Vol. 13, Pages 1487: Localization of SARS-CoV-2 Capping Enzymes Revealed by an Antibody against the nsp10 Subunit Viruses doi: 10.3390/v13081487 Authors: Vladimira Horova Barbora Landova Jan Hodek Karel Chalupsky Petra Krafcikova Dominika Chalupska Vojtech Duchoslav Jan Weber Evzen Boura Martin Klima Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the coronavirus disease-19 pandemic. One of the key components of the coronavirus replication complex are the RNA methyltransferases (MTases), RNA-modifying enzymes crucial for RNA cap formation. Recently, the structure of the 2’-O MTase has become available; however, its biological characterization within the infected cells remains largely elusive. Here, we report a novel monoclonal antibody directed against the SARS-CoV-2 non-structural protein nsp10, a subunit of both the 2’-O RNA and N7 MTase protein complexes. Using this antibody, we investigated the subcellular localization of the SARS-CoV-2 MTases in cells infected with the SARS-CoV-2.
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research