Improving mobilization of foreign DNA into < em > Zymomonas mobilis < /em > ZM4 by removal of multiple restriction systems

In this study we have developed an all-purpose strain, devoid of known host restriction systems and free of any antibiotic resistance markers, which dramatically improves the uptake efficiency of heterologous DNA into Z. mobilis ZM4. We also confirmed the role of a previously known restriction system as well as identified a previously unknown Type I RM system on an endogenous plasmid. Elimination of the barriers to DNA uptake as shown here will allow facile genetic engineering of Z. mobilis.PMID:34288704 | DOI:10.1128/AEM.00808-21

Source: Applied and Environmental Microbiology - July 21, 2021 Category: Microbiology Authors: Piyush Behari Lal Fritz Wells Kevin S Myers Rajdeep Banerjee Adam M Guss Patricia J Kiley Source Type: research