Sulfation of chitosan from < em > Sepia kobiensis < /em > as potential anticoagulant and antibacterial molecule

Nat Prod Res. 2021 Jul 24:1-7. doi: 10.1080/14786419.2021.1956492. Online ahead of print.ABSTRACTThe present work aimed to synthesis of chitin, chitosan and sulfation of chitosan from cuttlebone of cuttlefish Sepia kobiensis. Principally chitin was extracted through sequential processes of demineralisation and deproteinzation. Then chitosan was synthesized by a deacetylation and finally sulfated at semi-heterogeneous condition using chlorosulfonic acid in N,N-dimethylformamide. The synthesized macromolecules were characterized for its structural, physical and thermal (CHN, DDA, FT-IR, NMR, XRD, Viscometric analysis, SEM and DSC) properties. Apart from anticoagulant potential of the sulfated chitosan was tested using human plasma by means of activated partial thromboplastin time (APTT) and prothrombin time (PT). Further sulfated chitosan was tested for antibacterial potential by well diffusion method against eleven human pathogenic clinical isolates of both Gram positive and Gram-negative strains and minimum inhibitory concentrations (MIC) was calculated accordingly. The results of this study revealed the effectiveness of the sulfated chitosan at semi-heterogeneous conditions as a potent antibacterial and anticoagulant molecule.PMID:34304652 | DOI:10.1080/14786419.2021.1956492

Source: Natural Product Research - July 26, 2021 Category: Biochemistry Authors: Pasiyappazham Ramasamy Sivasankari Sekar Sivagurunathan Paramasivam Priyatharsini Suri Uma Chinnaiyan Rajesh Singh Bharath Prasad Tanguturi Raghavaiah Vidya Devanathadesikan Seshadri Source Type: research