Scrub typhus in Puducherry, India: Application of nested PCR targeting three different genes - 56 kDa, 47 kDa and groEL of < em > Orientia tsutsugamushi < /em > and comparison with ST IgM ELISA

CONCLUSION: Compared to IgM ELISA, sensitivity of three PCRs was 30, 51.43 and 61.43% for 56 kDa, 47 kDa and groEL targets, respectively. Since IgM ELISA positivity can persist up to one year, PCR confirms ST diagnosis in the acute phase of the illness, in the presence of IgM and even before IgM appears. Inclusion of all three genes - 56 kDa, 47 kDa and groEL, instead of a single 56 kDa target, identifies and confirms maximum number of ST patients.PMID:34290159 | DOI:10.4103/0972-9062.310866
Source: Journal of Vector Borne Diseases - Category: Infectious Diseases Authors: Source Type: research