Adaptation of < em > gltA < /em > and < em > ssrA < /em > assays for diversity profiling by Illumina sequencing to identify < em > Bartonella henselae, B. clarridgeiae < /em > and < em > B. koehlerae < /em >

Conclusion. The adaptation and multiplexing of existing PCR assays for diversity profiling via NGS is a feasible approach that is superior to traditional Sanger sequencing for Bartonella speciation and resolving mixed Bartonella infections. The adaptation of other PCR primers for Illumina NGS will be useful in future studies where mixed bacterial infections may be present.PMID:34296984 | DOI:10.1099/jmm.0.001400

Source: Journal of Medical Microbiology - July 23, 2021 Category: Microbiology Authors: Rosemonde Isabella Power Nichola Elisa Davies Calvani Yaarit Nachum-Biala Harold Salant Shimon Harrus Jan Šlapeta Source Type: research