Effect of fibulin-5 on aldosterone-induced apoptosis in human ascending aortic smooth muscle cells

Exp Ther Med. 2021 Aug;22(2):896. doi: 10.3892/etm.2021.10328. Epub 2021 Jun 23.ABSTRACTThe aim of the current study was to investigate the effect of aldosterone on apoptosis in human aortic smooth muscle cells (HA-VSMC) and to determine the role of fibulin-5 in the aldosterone-induced apoptosis of HA-VSMC cells. Through the construction of a fibulin-5 eukaryotic overexpression vector and a short hairpin RNA interference plasmid, fibulin-5 was overexpressed and silenced, respectively. The role of fibulin-5 in the aldosterone-induced apoptosis of HA-VSMC was subsequently determined. The overexpression of fibulin-5 inhibited the apoptosis of cells, particularly at low concentrations of aldosterone; a smaller effect on apoptosis was induced by high concentrations of aldosterone. fibulin-5 knockdown promoted the apoptosis of cells induced by high concentrations of aldosterone but had a smaller effect on the apoptosis of cells induced by low concentrations of aldosterone. Therefore, the results of the current study indicate that fibulin-5 inhibits the aldosterone-induced apoptosis of HA-VSMC cells and that this effect may be altered by changing the aldosterone concentration.PMID:34257709 | PMC:PMC8243313 | DOI:10.3892/etm.2021.10328

Source: Experimental and Therapeutic Medicine - July 14, 2021 Category: General Medicine Authors: Yongji Yan Xujie Liu Xuanchen Li Xuan Zhou Chaozhi Yang Jintao Tian Jun Pu Xiaoqun Niu Source Type: research