The complete mitochondrial genome of Choroterpes (Euthralus) yixingensis (Ephemeroptera: Leptophlebiidae) and its mitochondrial protein-coding gene expression under imidacloprid stress

Gene. 2021 Jul 15:145833. doi: 10.1016/j.gene.2021.145833. Online ahead of print.ABSTRACTAs one of the most common benthic invertebrates in freshwater, mayflies are very sensitive to changes in water quality and have high requirements for the water environment to allow their nymphs to successfully live and grow. Neonicotinoids, such as imidacloprid, can enter fresh water and pollute the aquatic environment. The present study had two goals: (1) investigate imidacloprid effects on mayfly larvae Choroterpes (Euthralus) yixingensis, and (2) contribute to the phylogenetic status of Ephemeroptera that has always been controversial. Nymphs were collected from Jinhua, China and exposed to different concentrations imidacloprid (5, 10, 20, and 40 μg/L) in the laboratory. Survival of C. yixingensis nymphs decreased as a function of time and imidacloprid concentration with only ∼55% survival after 72 h exposure to 40 μg/L imidacloprid. After culture under 40 μg/L imidacloprid for 24 h, the steady state transcript levels of mitochondrial COX3, ND4 and ND4L genes were reduced to just 0.07±0.11, 0.30±0.16, and 0.28±0.13 as compared with respective control values (P<0.01). Steady state transcript levels of ND4 and ND4L were also significantly reduced in a dose-dependent manner (P<0.05), suggesting that the steady state transcript pattern of these genes in mayfly nymphs can change in response to different levels of environmental contamination. Hence, the mitochondrial protein-co...
Source: Gene - Category: Genetics & Stem Cells Authors: Source Type: research