Global molecular epidemiology of carbapenem-resistant Escherichia coli (2002 –2017)

AbstractThe emergence of carbapenem-resistant (CR)Escherichia coli obliges an assessment of such strains ’ molecular epidemiology. Accordingly, we characterized in detail a globally distributed collection of CRE. coli isolates, then explored for associations between geographical origin and bacterial traits, and between different bacterial traits. We used established PCR-based assays and broth microdilution MIC determinations to characterize 343 global CR (i.e., non-susceptible to  ≥ 1 carbapenem) extraintestinalE. coli isolates (2002 –2017) for diverse molecular traits—including phylogroups, sequence types (STs), beta-lactamase genes, and 51 virulence genes—and susceptibility to 12 relevant antimicrobial agents. The study population was tremendously diverse according to all assessed variables. Nonetheless, certain geograph ically aligned, unifying themes emerged. These included an association of an Asia/West Pacific origin with non-B2/D/F phylogroups and STs, lower molecularly inferred virulence, more extensive resistance, and specific resistance genes (notably, metallo-beta-lactamases). Likewise, U.S. isolates from t he central region, vs. other regions, were more virulent-appearing and more often from phylogroup B2 and ST131, but less extensively resistant and more often carbapenemase-gene negative. The global CRE. coli population is highly diverse according to multiple characteristics and varies significantly by geographical region. This predictably will pose...
Source: European Journal of Clinical Microbiology and Infectious Diseases - Category: Microbiology Source Type: research