Extraction and purification of anthocyanins from < em > Peristrophe bivalvis < /em > (L.) < em > Merr. < /em > leaf (Acanthaceae) using aqueous two-phase systems

This study, therefore, focused on applying the aqueous two-phase system (ATPS), a green method for extracting and purifying anthocyanin in the leaf extract. The obtained results revealed that an ATPS composing from ethanol and ammonium sulfate with the concentrations of 32.5% and 25%, respectively, can recover 80% anthocyanin in leaf while eliminating 80% contaminated nitrogen compounds. Besides, the shelf-life of pigment in the purified extract was also elongated significantly in comparison with that in the crude extract.PMID:34254860 | DOI:10.1080/14786419.2021.1952203

Source: Natural Product Research - July 13, 2021 Category: Biochemistry Authors: Phuong H Le Duy N Dao Tin Q Huynh Tra T T Tran Viet Nguyen Source Type: research