Comparison of different protocols of RNA preparation from circulating blood for RNA sequencing

CONCLUSIONS: The quality of RNA extracted from circulating blood is affected by high-speed centrifugation and cryopreservation. Adding an RNA stabilizer during the cryopreservation of circulating blood significantly improved RNA quality and quantity. The quality of extracted RNA from circulating blood is improved when using TRIzol relative to that attained with a commercial kit.PMID:34173130 | DOI:10.1007/s10529-021-03152-8
Source: Biotechnology Letters - Category: Biotechnology Authors: Source Type: research