Evaluation of DNA double-strand break repair capacity in human cells: critical overview of current functional methods

Publication date: Available online 25 June 2021Source: Mutation Research/Reviews in Mutation ResearchAuthor(s): Xavier Tatin, Giovanna Muggiolu, Sylvie Sauvaigo, Jean Breton
Source: Mutation Research Reviews in Mutation Research - Category: Genetics & Stem Cells Source Type: research

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This article describes a novel technique for primary cleft lip repair that allows for the formation of the Cupid's bow peak without scarring.Plastic and Reconstructive Surgery
Source: Medscape Today Headlines - Category: Consumer Health News Tags: Plastic Surgery & Aesthetic Medicine Journal Article Source Type: news
Oncol Rep. 2021 Nov;46(5):235. doi: 10.3892/or.2021.8186. Epub 2021 Sep 15.ABSTRACTCholangiocarcinoma (CCA) is a highly aggressive malignant tumor with an extremely poor prognosis. Minichromosome maintenance 8 homologous recombination repair factor (MCM8) is a helicase involved in the elongation step of DNA replication and tumorigenesis. In the present study, the clinical significance and biological function of MCM8 in CCA were investigated. The expression levels of MCM8 in CCA and paracancerous tissues were analyzed using immunohistochemical staining. The potential mechanisms underlying MCM8 and the biological effects of ...
Source: Oncology Reports - Category: Cancer & Oncology Authors: Source Type: research
This study aims to evaluate the potential effects of rFGF21 on cerebrovascular remodeling after T2DM stroke. Permanent distal middle cerebral artery occlusion was performed in heterozygous non-diabetic db/ + and homozygous diabetic db/db mice. Daily rFGF21 administration was initiated 1 week after stroke induction and maintained for up to 2 weeks thereafter. Multiple markers associated with post-stroke recovery, including angiogenesis, oligodendrogenesis, white matter integrity, and neurogenesis, were assessed up to 3 weeks after stroke. Our results showed an impairment in post-stroke vascular remodeling under T2DM conditi...
Source: Cell Research - Category: Cytology Authors: Source Type: research
Mol Cancer Res. 2021 Sep 14:molcanres.MCR-21-0093-A.2021. doi: 10.1158/1541-7786.MCR-21-0093. Online ahead of print.ABSTRACTStrengthened DNA repair pathways in tumor cells contribute to the development of resistance to DNA-damaging agents. Consequently, targeting proteins in these pathways is a promising strategy for tumor chemosensitization. Here, we show that the expression of a subset of Fanconi anemia (FA) genes is attenuated in glioblastoma tumor cells deficient in Methylthioadenosine Phosphorylase (MTAP), a common genetic alteration in a variety of cancers. Subsequent experiments in cell line models of different canc...
Source: Cell Research - Category: Cytology Authors: Source Type: research
Curr Med Chem. 2021 Sep 13. doi: 10.2174/0929867328666210914115411. Online ahead of print.ABSTRACTIn recent years, knowledge of cardiac regeneration mechanisms has dramatically expanded. Regeneration can replace lost parts of organs, common among animal species. The heart is commonly considered an organ with terminal development, which has no reparability potential during post-natal life; however, some intrinsic regeneration capacity has been reported for cardiac muscle, which opens novel avenues in cardiovascular disease treatment. Different endogenous mechanisms were studied for cardiac repairing and regeneration in rece...
Source: Current Medicinal Chemistry - Category: Chemistry Authors: Source Type: research
Int J Med Sci. 2021 Jul 25;18(15):3361-3366. doi: 10.7150/ijms.62903. eCollection 2021.ABSTRACTFerroptosis is an iron-dependent form of regulated cell death, which is characterized by a large amount of lipid peroxide accumulation and the imbalance of redox state in cells. Ferroptosis is usually accompanied with the dysfunction of lipid repair enzyme (glutathione peroxidase 4, GPX4), large masses of iron accumulation and lipid peroxidation of polyunsaturated fatty acids (PUFAs). Ferroptosis is related to several signaling pathways, including amino acid and iron metabolism, ferritinophagy, cell adhesion and p53 and Keap1/Nrf...
Source: International Journal of Medical Sciences - Category: Biomedical Science Authors: Source Type: research
Cytokemia is associated with microcirculatory alterations often with persistent loss of coherence between the micro- and macrocirculation, linked to organ failure and poor outcome of septic patients. Addition of a hemoadsorbant filter to an extracorporeal circuit next to conventional treatment of septic shock results in the hematological clearance of cytokines, hypothetically leading to normalization of the microcirculation and thus organ perfusion. Bedside sublingual microcirculatory assessment using handheld vital microscopy allows real-time direct visualization of the microcirculation and its response to therapy. This i...
Source: Blood Purification - Category: Hematology Source Type: research
Analyst, 2021, Accepted Manuscript DOI: 10.1039/D1AN01088C, PaperGrace Sarabia, Bhavya Sharma Increased acidification of aquatic habitat due to climate change is damaging mollusks. Non-destructive methods for analysis are necessary to study these endangered species. We analyzed five Unionidae gastropods using Raman... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Analyst latest articles - Category: Chemistry Authors: Source Type: research
Contributors : Jane J Xu ; Alistair M Chalk ; Iva Nikolic ; Kaylene J Simpson ; Monique F Smeets ; Carl R WalkleySeries Type : OtherOrganism : Mus musculusCurrent strategies to target RNA splicing mutant myeloid cancers propose disruption of normal splicing activity by targeting the splicing apparatus therapeutically. This approach is only modestly sensitizing and is also toxic to non-mutant bearing wild-type cells. To explore potentially exploitable genetic interactions with spliceosome mutations, we combined data mining and functional screening for synthetic lethal interactions with an Srsf2P95H/+ mutation. Analysis of m...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Other Mus musculus Source Type: research
Contributors : Jane J Xu ; Alistair M Chalk ; Monique F Smeets ; Carl R WalkleySeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusCurrent strategies to target RNA splicing mutant myeloid cancers propose disruption of normal splicing activity by targeting the splicing apparatus therapeutically. This approach is only modestly sensitizing and is also toxic to non-mutant bearing wild-type cells. To explore potentially exploitable genetic interactions with spliceosome mutations, we combined data mining and functional screening for synthetic lethal interactions with an Srsf2P95H/+ mutation. An...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
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