Comparison of < sup > 1 < /sup > H-MRS, Dixon fat-water separation and Z-spectral imaging for quantification of brown adipose tissue in rats

Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):783-788. doi: 10.12122/j.issn.1673-4254.2021.05.21.ABSTRACTOBJECTIVE: To evaluate the performance of 1H-magnetic resonance spectroscopy (1H-MRS), Dixon fat-water separation and Z-spectral magnetic resonance imaging (ZS-MRI) for quantification of fat content in phantoms and brown adipose tissues in rats.OBJECTIVE: First, six water-oil mixture phantoms with different fat fractions (0, 20%, 40%, 60%, 80% and 100%) were prepared and placed in a 50-mL centrifuge tube. ZS-MRI, 1H-MRS and Dixon's method were used to quantitatively evaluate the fat content of the phantom, and the results were compared against the actual fat fractions. Then, ZS-MRI and Dixon's method were used to collect the data in the interscapular region of 6 rats, the fat-water distribution map was calculated, and the results were compared with 1H-MRS.OBJECTIVE: ZS-MRI accurately quantified fat contents in the phantoms (Y=0.95*X+1.48). ZS-MRI was capable of distinguishing brown adipose tissue from white adipose tissue and defining the spatial distribution of the adipose tissue, and the results were highly consistent with those obtained by Dixon's method. No significant differences were found in the results derived by ZS-MRI and 1H-MRS for quantification of brown adipose tissue (P=0.35).OBJECTIVE: ZS-MRI can generate an artifact-free fat distribution map for quantitative measurement of the content and distribution of brown adipose tissues in rats.PMID:34134968 | DOI:...
Source: Journal of Southern Medical University - Category: Universities & Medical Training Authors: Source Type: research