CTPS forms the cytoophidium in zebrafish

Exp Cell Res. 2021 Jun 12:112684. doi: 10.1016/j.yexcr.2021.112684. Online ahead of print.ABSTRACTCytidine triphosphate synthase (CTPS) catalyzes the rate-limiting step of de novo CTP biosynthesis. An intracellular structure of CTPS, the cytoophidium, has been found in many organisms including prokaryotes and eukaryotes. Formation of the cytoophidium has been suggested to regulate the activity and stability of CTPS and may participate in certain physiological events. Herein, we demonstrate that both CTPS1a and CTPS1b in zebrafish are able to form the cytoophidium in cultured cells. A point mutation, H355A, abrogates cytoophidium assembly of zebrafish CTPS1a and CTPS1b. In addition, we show the presence of CTPS cytoophidia in multiple tissues of larval and adult fish under normal conditions, while treatment with a CTPS inhibitor 6-diazo-5-oxo-L-norleucine (DON) can induce more cytoophidia in some tissues. Our findings reveal that forming the CTPS cytoophidium is a natural phenomenon of zebrafish and provide valuable information for future research on the physiological importance of this intracellular structure in vertebrates.PMID:34129847 | DOI:10.1016/j.yexcr.2021.112684

Source: Experimental Cell Research - June 15, 2021 Category: Cytology Authors: Chia-Chun Chang Gerson Dierley Keppeke Christopher L Antos Min Peng Luis Eduardo Coelho Andrade Li-Ying Sung Ji-Long Liu Source Type: research