Testosterone induces leukocyte migration by NADPH oxidase-driven ROS- and COX2-dependent mechanisms

The mechanisms whereby testosterone increases cardiovascular risk are not clarified. However, oxidative stress and inflammation seem to be determinant. Herein, we sought to determine whether exogenous testosterone, in physiological levels, induces leukocyte migration, a central feature in immune and inflammatory responses, and the mediating mechanisms. We hypothesized that testosterone induces leukocyte migration via NADPH oxidase (NADPHox)-driven reactive oxygen species and cyclooxygenase-dependent mechanisms. Sixteen week-old Wistar rats received an intraperitoneal injection (5 mL) of either testosterone (10-7 mol/L) or saline. Rats were pre-treated with 5 mL of sodium salicylate (SS, non-selective COX inhibitor, 1.25x10-3 mol/L, 1h prior to testosterone or saline), flutamide (androgen receptor antagonist, 10-5 mol/L), apocynin (NADPHox inhibitor, 3x10-4 mol/L), NS398 (COX2 inhibitor, 10-4 mol/L) or saline, 4h before testosterone or saline administration. Leukocyte migration was assessed 24 hours after testosterone administration by intravital microscopy of the mesenteric bed. Serum levels of testosterone were measured by radioimmunoassay. NADPHox activity was assessed in membrane fractions of the mesenteric bed by dihydroethidium fluorescence and in isolated VSMC by HPLC. NADPHox subunits and VCAM expression were determined by immunoblotting. Testosterone administration did not change serum levels of endogenous testosterone, but increased venular leukocyte migration to the...
Source: Clinical Science - Category: Biomedical Science Authors: Source Type: research

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Authors: Stange EF, Schroeder BO Abstract Introduction: Inflammatory bowel diseases (IBD) are on the rise worldwide. This review covers the current concepts of the etiology of Crohn´s disease and ulcerative colitis by focusing on an unbalanced interaction between the intestinal microbiota and the mucosal barrier. Understanding these issues is of paramount importance for the development of targeted therapies aiming at the disease cause. Area covered: Gut microbiota alterations and a dysfunctional intestinal mucosa are associated with IBD. Here we focus on specific defense structures of the mucosal barrier, nam...
Source: Expert Review of Gastroenterology and Hepatology - Category: Gastroenterology Tags: Expert Rev Gastroenterol Hepatol Source Type: research
ConclusionsOromotor exercises are more effective than incentive spirometry training in improving both pulmonary and oromotor functions in children with Down's syndrome.
Source: Journal of Taibah University Medical Sciences - Category: Universities & Medical Training Source Type: research
Publication date: Available online 11 October 2019Source: Journal of Taibah University Medical SciencesAuthor(s): Beuy Joob, Viroj Wiwanitkit
Source: Journal of Taibah University Medical Sciences - Category: Universities & Medical Training Source Type: research
. Got my question answered, thanks.
Source: Student Doctor Network - Category: Universities & Medical Training Authors: Tags: General Residency Issues Source Type: forums
I'm having a hard time finding an interview suit that is safe enough yet also allows me to express my fashion forward sense. Do y'all think any of these suits would work and if so, which one? I would go with a neutral color blouse and black pumps or oxfords. Opinions please! Thanks. 1. 2. 3. 4. 5. 6... How do we feel about any of these Zara suits for interviews?
Source: Student Doctor Network - Category: Universities & Medical Training Authors: Tags: Pre-Medical - MD Source Type: forums
Publication date: Available online 11 October 2019Source: The SurgeonAuthor(s): Gregory L. Falk
Source: The Surgeon - Category: Surgery Source Type: research
ConclusionsPatients receiving perioperative gabapentin, acetaminophen, and NSAIDs under an ERAS protocol required significantly fewer narcotics and shorter length of stay. This protocol may merit consideration for use at other centers.
Source: The American Journal of Surgery - Category: Surgery Source Type: research
ConclusionsMore than half (51%) of women who underwent genetic counseling and received an uninformative negative genetic test result had a significantly elevated risk for the development of breast cancer. It is, therefore, imperative that women do not conclude that a negative genetic test result represents a lack of risk.
Source: The American Journal of Surgery - Category: Surgery Source Type: research
Publication date: Available online 11 October 2019Source: The American Journal of SurgeryAuthor(s): Dhruv J. Patel, Waseem Lutfi, Patrick Sweigert, Emanuel Eguia, Gerard Abood, Lawrence Knab, Paul C. Kuo, Marshall S. BakerAbstractBackgroundPrior studies of adjuvant systemic therapy in pancreatic acinar cell carcinoma have been underpowered.MethodsWe queried the National Cancer Data Base to identify patients presenting with resectable (clinical stage I and II) acinar cell carcinoma between 2004 and 2015. Multivariable Cox Regression was used to evaluate the association between overall survival and systemic therapy.Results29...
Source: The American Journal of Surgery - Category: Surgery Source Type: research
ConclusionOur pilot curriculum addresses a gap in resident education about surgical cost. Residents generally regard equipment standardization as either improving or not changing hospital metrics.
Source: The American Journal of Surgery - Category: Surgery Source Type: research
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