Lysinibacillus agricola sp. nov., isolated from soil

Arch Microbiol. 2021 Jun 1. doi: 10.1007/s00203-021-02394-4. Online ahead of print.ABSTRACTA gram-staining-positive, rod-shaped bacterium, designed strain FJAT-51161T was isolated from farmland soil collected from Fujian Province, China. Growth was observed at 25-40 °C (optimum 30 °C), pH 7.0-9.0 (optimum 7.0), and NaCl tolerance in the range of 0-7% (w/v), respectively. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain FJAT-51161T belonged to the genus Lysinibacillus, and had the closest relationship with Lysinibacillus xylanilyticus XDB9T (99.0% 16S rRNA sequence similarity). The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values based on the genome sequence analysis between strain FJAT-51161T and the closest reference strain were 38.0% for dDDH and 88.7% for ANI, respectively, lower than the prokaryotic species delineation values. Further analysis showed that strain FJAT-51161T shared the fatty acid profiles such as iso-C15:0 (46.7%), iso-C16:0 (15.8%), C16:1 ω7c alcohol (14.0%), anteiso-C15:0 (6.9%) with other members of the genus Lysinibacillus. As the peptidoglycan contained the amino acids alanine, lysine, glycine and aspartic acid, the type A4α was deduced as found in the closest relatives of strain FJAT-51161T. The peptidoglycan of strain FJAT-51161T was L-Lys-D-Asp (type A4α). The main quinone was MK-7 and MK-6. The major polar lipids were diphosphatidylglycerol (DPG) and phosphatidylethanolamine...
Source: Archives of Microbiology - Category: Microbiology Authors: Source Type: research