Comparison of seven circulating microRNAs and ALT in carbon tetrachloride –induced liver injury model in Macaca fascicularis

This study was initiated to determine the potential of circulating miR-122, miR-192, miR-885, miR-151, miR-378, miR-365, and miR-31 as a biomarker for carbon tetrachloride (CCl4) –induced liver injury inMacaca fascicularis. The tenMacaca fascicularis were orally given 15% CCl4 (10 mL/kg) to establish the DILI. The serum at 48 h after administration when a single hepatocyte showed histopathological necrosis and vacuolation was isolated and quantified for serum miRNAs by next-generation sequencing (NGS) analysis. It was revealed that miR-122, miR-192, miR-885, miR-151, miR-378, miR-365, miR-31, and miR-802 were upregulated in the circulating serum at 48 h. The serum in different time points was collected to analyze the levels of alanine aminotransferase (ALT) and the eight miRNAs. The concentration of ALT was detected by automatic biochemistry analyzer and the miRNA level was quantified by real-time reverse-transcription PCR. All quantitative PCR values were normalized to inter-control miR-39 and calculated with the comparative Ct method. The miR-802 was not detected at each time point in the following real-time PCR amplification because of unknown reasons and would not involve any other comparison analysis in the present study. Serum ALT increased significantly from 24 h after CCl4-induced liver injury and reached top value at 48 h. The increasing fold changes of miR-122 were more than 8-fold from 2 h after administration and that of miR-885 were over 5-fold from 6 h. The to...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research