Effect of vitamins and metal ions on productivity and charge heterogeneity of IgG1 expressed in CHO cells
This study aims to explore the impact of vitamins and metal ions on protein expression and post-translational modifications specifically charge heterogeneity. Biotin, choline chloride, D-calcium pantothenate, folic acid, pyridoxine hydrochloride, thiamine hydrochloride vitamins and Fe, Cu, Mg, Co, Zn, Mn, Ni metal ions were examined in this study. The results indicate that pyridoxine enhances productivity while Zn, Cu, Fe, Mn, and biotin impact charge heterogeneity. While, Fe, Mn and Ni enhance production of the acidic variants, Cu and biotin inhibit it. Zn reduces formation of basic variants while biotin enhances it. The results from this investigation could be used for process control so as to get consistent charge variant profile, in particular for biosimilars.PMID:34028198 | DOI:10.1002/biot.202000464
Source: Biotechnology Journal - Category: Biotechnology Authors: Neelesh Gangwar Rishabh Mishra Niharika Budholiya Anurag S Rathore Source Type: research
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