Supplementation of critical amino acids improves glycerol and lactose uptake and enhances recombinant protein production in Escherichia coli

CONCLUSIONS AND IMPLICATIONS: We hypothesize that supplementation of critical AAs serves dual purpose: i) faster assimilation of carbon sources, and ii) combating metabolic stress arises due to AAs starvation. The substrate uptake and gene expression profiles demonstrate that AAs addition enhances glycerol and lactose assimilation due to overall improvement in their metabolism governed by global regulators of carbon metabolism. This article is protected by copyright. All rights reserved.PMID:34047499 | DOI:10.1002/biot.202100143
Source: Biotechnology Journal - Category: Biotechnology Authors: Source Type: research