Crystallization and preliminary crystallographic analysis of acetophenone reductase from Geotrichum candidum NBRC 4597

Acetophenone reductase (APRD) from Geotrichum candidium NBRC 4597 was crystallized by the hanging-drop vapour-diffusion method using PEG 3350 as a precipitant. The crystal belonged to space group P6522, with unit-cell parameters a = b = 104.5, c = 273.7 Å, and diffracted to 2.6 Å resolution. Phasing using the single-wavelength anomalous diffraction method was successful. Model building and crystallographic refinement are in progress.

Source: Acta Crystallographica Section F - February 19, 2015 Category: Biochemistry Authors: Sugiyama, Y.Senda, M.Senda, T.Matsuda, T. Tags: acetophenone reductase enantioselectivity asymmetric reduction research communications Source Type: research