Crystallization and preliminary X-ray crystallographic analysis of human myotubularin-related protein 1

In this study, human MTMR1 was overexpressed in Escherichia coli, purified and crystallized at 277 K using polyethylene glycol 20 000 as a precipitant. Diffraction data were collected to 2.0 Å resolution using synchrotron radiation. The crystals belonged to space group P1, with unit-cell parameters a = 67.219, b = 96.587, c = 97.581 Å, α = 87.597, β = 86.072, γ = 77.327°. Assuming the presence of four molecules in the asymmetric unit, the calculated Matthews coefficient value was 2.61 Å3 Da−1 and the corresponding solvent content was 52.9%.

Source: Acta Crystallographica Section F - February 19, 2015 Category: Biochemistry Authors: Bong, S.M.Yang, S.W.Choi, J.-W.Kim, S.J.Lee, B.I. Tags: myotubularin MTMR phosphatidylinositol phosphatase research communications Source Type: research