Transcriptome landscape of double negative T cells by single-cell RNA sequencing

In conclusion, we provide a map of the heterogeneity in naïve and active DNT cells, addresses the controversy about DNT cells, and provides potential transcription signatures of DNT cells. The landscape approach herein will eventually become more feasible through newer high throughput methods and will enable clustering data to be fed into a systems analysis approach. Thus the approach should become the "backdrop" of similar studies in the myriad murine models of autoimmunity, potentially highlighting the importance of DNT cells and other minor lineage of cells in immune homeostasis. The clear characterization of functional DNT subsets into helper DNT, cytotoxic DNT and innate DNT will help to better understand the intrinsic roles of different functional DNT subsets in the development and progression of autoimmune diseases and transplant rejection, and thereby may facilitate diagnosis and therapy.PMID:34022742 | DOI:10.1016/j.jaut.2021.102653

Source: Journal of Autoimmunity - May 22, 2021 Category: Allergy & Immunology Authors: Lu Yang Yanbing Zhu Dan Tian Song Wang Jincheng Guo Guangyong Sun Hua Jin Chunpan Zhang Wen Shi M Eric Gershwin Zhongtao Zhang Yi Zhao Dong Zhang Source Type: research