Genes involved in phosphatidylcholine biosynthesis correlate with nuclear factor- κB in biliary tract cancer patients: Evidence from < sup > 1 < /sup > H NMR and computational analyses
In this study, we measured the clinicopathological features in 40 cases of histologically confirmed GBC and 16 cases of chronic cholecystitis (CC). The female to male ratio in the GBC and CC groups were 3.44:1 and 2.2:1, respectively. The GBC patients exhibited well to poorly differentiated tumor. In the CC group, all patients showed cholecystitis with no evidence of dysplasia or malignancy. The majority of GBC and CC patients reported pain. Using 1H NMR spectroscopy, we observed 4-folds increase in the level of choline containing phospholipids (CCPLs) in the gallbladder of GBC patients as compared to CC patients. Other lipid metabolites such as cholesterol ester, C18-cholesterol and saturated fatty acids were insignificantly changed between GBC and CC patients. Moreover, the level of CCPLs in the GBC patients with BMI <25 kg/m2 was significantly higher as compared to CC patients. Further, a significant increase in the CCPLs level was observed in GBC female patients in comparison to CC patients. From the computational analyses, we observed that the genes involved in the biosynthesis of phosphatidylcholine (PtdCho) indirectly interact with the RELA, which encodes the NF-κB p65 subunit. The genes involved in the PtdCho biosynthesis were also correlated with the overall and disease-free survival of cholangiocarcinoma patients. The study opens new window for exploring the diagnostic and therapeutic potential of CCPLs in GBC patients.PMID:34023500 | DOI:10.1016/j.bbalip.2021.1...
Source: Mol Biol Cell - Category: Molecular Biology Authors: Anusmita Shekher Amit Kumar Tiwari Nikee Awasthee Sumit Singh Verma Vinod Kumar Dixit Neeraj Sinha Subash Chandra Gupta None Puneet Source Type: research
More News: Bile Duct Cancer | Cancer | Cancer & Oncology | Cholangiocarcinoma | Cholesterol | Chronic Pain | Gallbladder Cancer | Gastroenterology | Genetics | Molecular Biology | Pain | Study