Genetic analysis of plant endophytic Pseudomonas putida BP25 and chemo-profiling of its antimicrobial volatile organic compounds

Publication date: Available online 17 February 2015 Source:Microbiological Research Author(s): Neelam Sheoran , Agisha Valiya Nadakkakath , Vibhuti Munjal , Aditi Kundu , Kesavan Subaharan , Vibina Venugopal , Suseelabhai Rajamma , Santhosh J. Eapen , Aundy Kumar Black pepper associated bacterium BP25 was isolated from root endosphere of apparently healthy cultivar Panniyur-5 that protected black pepper against Phytophthora capsici and Radopholus similis -the major production constraints. The bacterium was characterized and mechanisms of its antagonistic action against major pathogens elucidated. The polyphasic phenotypic analysis revealed its identity as Pseudomonas putida. Multi Locus Sequence Typing revealed that the bacterium shared gene sequences with several other isolates representing diverse habitats. Tissue localization assays exploiting green fluorescence protein expression clearly indicated that PpBP25 endophytically colonized not only its host plant -black pepper, but also other distantly related plants such as ginger and arabidopsis. PpBP25 colonies could be enumerated from internal tissues plants four weeks post inoculation indicated its stable establishment and persistence in the plant system. The bacterium inhibited broad range of pathogens such as Phytophthora capsici, Pythium myriotylum, Giberella moniliformis, Rhizoctonia solani, Athelia rolfsii, Colletotrichum gloeosporioides and plant parasitic nematode, Radopholus similis by its volatile sub...
Source: Microbiological Research - Category: Infectious Diseases Source Type: research