Nocathioamides, uncovered by a Tunable Metabologenomic Approach, define a Novel Class of Chimeric Lanthipeptides

Angew Chem Int Ed Engl. 2021 May 14. doi: 10.1002/anie.202102571. Online ahead of print.ABSTRACTThe increasing number of available genomes, in combination with advanced genome mining techniques, unveiled a plethora of biosynthetic gene clusters (BGCs) coding for ribosomal synthesized and post-translationally modified peptides (RiPPs). The products of these BGCs often represent an enormous resource for new and bioactive compounds, but frequently, they cannot be readily isolated and remain cryptic. Here, we describe a tunable metabologenomic approach that recruits a synergism of bioinformatics in tandem with isotope- and NMR-guided platform to identify the product of an orphan RiPP gene cluster in the genomes of Nocardia terpenica IFM 0406 and 0706T. The application of this tactic resulted in the discovery of nocathioamides family as a founder of a new class of chimeric lanthipeptides I.PMID:33991039 | DOI:10.1002/anie.202102571

Source: Angewandte Chemie - May 15, 2021 Category: Chemistry Authors: Hamada Saad Saefuddin Aziz Matthias Gehringer Markus Kramer Jan Straetener Anne Berscheid Heike Br ötz-Oesterhelt Harald Gross Source Type: research