Genes, Vol. 12, Pages 746: The Seed Development Factors TT2 and MYB5 Regulate Heat Stress Response in Arabidopsis
Genes, Vol. 12, Pages 746: The Seed Development Factors TT2 and MYB5 Regulate Heat Stress Response in Arabidopsis
Genes doi: 10.3390/genes12050746
Authors:
Pierre Jacob
Gwilherm Brisou
Marion Dalmais
Johanne Thévenin
Froukje van der Wal
David Latrasse
Ravi Suresh Devani
Moussa Benhamed
Bertrand Dubreucq
Adnane Boualem
Loic Lepiniec
Richard G. H. Immink
Heribert Hirt
Abdelhafid Bendahmane
HEAT SHOCK FACTOR A2 (HSFA2) is a regulator of multiple environmental stress responses required for stress acclimation. We analyzed HSFA2 co-regulated genes and identified 43 genes strongly co-regulated with HSFA2 during multiple stresses. Motif enrichment analysis revealed an over-representation of the site II element (SIIE) in the promoters of these genes. In a yeast 1-hybrid screen with the SIIE, we identified the closely related R2R3-MYB transcription factors TT2 and MYB5. We found overexpression of MYB5 or TT2 rendered plants heat stress tolerant. In contrast, tt2, myb5, and tt2/myb5 loss of function mutants showed heat stress hypersensitivity. Transient expression assays confirmed that MYB5 and TT2 can regulate the HSFA2 promoter together with the other members of the MBW complex, TT8 and TRANSPARENT TESTA GLABRA 1 (TTG1) and that the SIIE was involved in this regulation. Transcriptomic analysis revealed that TT2/MYB5 target promoters were enriched in SIIE. Overall, we report a new function of TT2 and MYB5 in stress resistance and a role in SIIE-mediat...
Source: Genes - Category: Genetics & Stem Cells Authors: Pierre Jacob Gwilherm Brisou Marion Dalmais Johanne Th évenin Froukje van der Wal David Latrasse Ravi Suresh Devani Moussa Benhamed Bertrand Dubreucq Adnane Boualem Loic Lepiniec Richard G. H. Immink Heribert Hirt Abdelhafid Bendahmane Tags: Article Source Type: research