Molecules, Vol. 26, Pages 2912: A Multimethodological Characterization of Cannabis sativa L. Inflorescences from Seven Dioecious Cultivars Grown in Italy: The Effect of Different Harvesting Stages

Molecules, Vol. 26, Pages 2912: A Multimethodological Characterization of Cannabis sativa L. Inflorescences from Seven Dioecious Cultivars Grown in Italy: The Effect of Different Harvesting Stages Molecules doi: 10.3390/molecules26102912 Authors: Mattia Spano Giacomo Di Matteo Cinzia Ingallina Bruno Botta Deborah Quaglio Francesca Ghirga Silvia Balducci Silvia Cammarone Enio Campiglia Anna Maria Giusti Giuliana Vinci Mattia Rapa Salvatore Ciano Luisa Mannina Anatoly P. Sobolev The chemical profile of the female inflorescence extracts from seven Cannabis sativa L. dioecious cultivars (Carmagnola, Fibranova, Eletta Campana, Antal, Tiborszallasi, Kompolti, and Tisza) was monitored at three harvesting stages (4, 14, and 30 September), reaching from the beginning of flowering to end of flowering/beginning of seed formation, using untargeted nuclear magnetic resonance (NMR) and targeted (ultra-high-performance liquid chromatography (UHPLC) and spectrophotometry) analyses. The tetrahydrocannabinol content was always below the legal limits (<0.6%) in all the analyzed samples. The NMR metabolite profile (sugars, organic acids, amino acids, and minor compounds) subjected to principal components analysis (PCA) showed a strong variability according to the harvesting stages: samples harvested in stage I were characterized by a high content of sucrose and myo-inositol, whereas the ones harvested in stage II showed high levels of succinic acid,...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research