Nanodisc reconstitution of flavin mononucleotide binding domain of cytochrome-P450-reductase enables high-resolution NMR probing

Chem Commun (Camb). 2021 May 14;57(39):4819-4822. doi: 10.1039/d1cc01018b. Epub 2021 Apr 16.ABSTRACTCytochrome-P450-reductase transfers electrons to cytochrome-P450 through its flavin mononucleotide binding domain (FBD). Despite the importance of membrane-anchoring for FBD function, studies have focused on its soluble domain lacking the transmembrane-domain. Here we demonstrate that the reconstitution of FBD in nanodiscs enables high-resolution NMR measurements and renders a stable conformation.PMID:33982687 | DOI:10.1039/d1cc01018b
Source: Chemical Communications - Category: Chemistry Authors: Source Type: research