Characterization of Siphoviridae phage Z and studying its efficacy against multi-drug resistant (MDR) Klebsiella pneumoniae planktonic cells and biofilm.

Characterization of Siphoviridae phage Z and studying its efficacy against multi-drug resistant (MDR) Klebsiella pneumoniae planktonic cells and biofilm. J Med Microbiol. 2015 Feb 13; Authors: Jamal M, Hussain T, Das CR, Andleeb S Abstract Biofilm is involved in many serious consequences for public health and is a major virulence factor contributing to the chronicity of infections. The aim of the current study was to isolate and characterize a bacteriophage that inhibit multi-drug resistant Klebsiella pneumonia (M) in palnktonic form as well as biofilm. This phage designated as bacteriophage Z was isolated from waste water. Its adsorption rate to its host bacterium was significantly enhanced by MgCl2 and CaCl2. It has a wide range of pH and heat stability. From its one step growth, latent time and burst size was determined that were 24 min and about 320 virions per cell, respectively. As analyzed by transmission electron microscopy, phage Z had a head of width (76±10 nm) and length of (92±14 nm) with an icosahedrons' sides of 38 nm with a non- contractile (200±15 nm) long and (14-29 nm) wide tail belonging to family Siphoviridae of order Caudovirales. Six structural proteins ranging from 18 to 65 kDa were revealed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Its genome was found to be comprised of double stranded DNA with approximate size of 36 kb. Bacteria were grown in suspension and as biofilms to co...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research