Protective effect of acute splenic irradiation in rats with traumatic brain injury

Objective To explore the protective effect of acute splenic irradiation against traumatic brain injury (TBI) in rats. Methods A rat model of TBI was established according to Feeney’s method. Splenic irradiation was performed by the reverse intensity-modulated radiation therapy (IMRT) source-axis distance (SAD) irradiation technique. Rat brain tissue samples were collected, the water content of the rat brain tissue was determined and the abundance of microglia was detected by immunofluorescence. Spleens were collected to measure the spleen index. Lung, liver, small intestine and kidney tissues were taken for hematoxylin and eosin staining to observe whether there was radiation-induced pathological damage. Peripheral blood was collected to detect tuftsin and the inflammatory factors IL-6 and IL-10. Results Compared with the nonirradiated TBI rat group, the 4-h spleen irradiation TBI rat group showed (1) increased behavioral scores at 3 days after TBI (P 
Source: NeuroReport - Category: Neurology Tags: Degeneration and Repair Source Type: research