GSE173808 The transcriptome of paired major and minor salivary gland tissue in patients with primary Sj ögren's syndrome

Contributors : Gwenny M Verstappen ; Lu Gao ; Sarah Pringle ; Erlin A Haacke ; Bert van der Vegt ; Silvia C Liefers ; Vishal Patel ; Yanhua Hu ; Sumanta Mukherjee ; Julie Carman ; Laurence Menard ; Frederik K Spijkervet ; Arjan Vissink ; Hendrika Bootsma ; Frans G KroeseSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensWhile all salivary glands (SGs) can be involved in primary Sj ögren’s syndrome (pSS), their respective role in pathogenesis remains unclear. To assess immunopathway activation in paired parotid and labial gland tissue from biopsy-positive and biopsy-negative pSS and non-SS sicca patients, paraffin-embedded, paired parotid and labial salivary gland tissue an d peripheral blood mononuclear cells were obtained from 39 pSS and 20 non-SS sicca patients. The patients were subdivided based on fulfillment of ACR-EULAR criteria and histopathology and the samples were analyzed for differentially expressed genes (DEGs). The principal component analysis of SG gen e expression could only separate biopsy-positive pSS from non-SS sicca patients. However, when comparing the transcriptome of biopsy-positive pSS and biopsy-negative non-SS sicca patients, 1235 and 624 DEGs (FDR1) were identified for parotid and labial glands, respectively. The number of DEGs between biopsy negative pSS and non-SS sicca patients was scarce. The overall, transcript expression levels correlated strongly between parotid and labial glands (R2=0.86, p ...

Source: GEO: Gene Expression Omnibus - May 5, 2021 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research